Product Description: ubiquitin carboxyl-terminal hydrolase UCH54
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.19.12 (Ubiquitinyl hydrolase 1)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0019784 | NEDD8-specific protease activity |
| Function | GO:0004843 | thiol-dependent deubiquitinase |
| Process | GO:0000338 | protein deneddylation |
| Process | GO:0016579 | protein deubiquitination |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.10 | 0.95 |
| Trophozoite | expressed | 0.74 | 0.59 |
| Schizont | expressed | 0.44 | 0.74 |
| Gametocyte | expressed | 0.95 | 0.50 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1117100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -2.89 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0930900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0930900)
Modification: Disrupted | RMgm-2628
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIJ6
"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=3.97) with 6QE6 (Global RMSD=19.71)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.19.12 | ubiquitinyl hydrolase 1 | KClNEMDTTEDTAEGTARNAisiRNApeptidesAntibodyubiquitinLDN-574442-propenal... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q92560 | Ubiquitin carboxyl-terminal hydrolase BAP1 | Homo sapiens | OrthoDB | MLS000083189 |
Ortholog Group (OrthoMCL): OG6_102753
Most Similar Human Ortholog: Q5LJB0
TM-align score: 0.86 | RMSD: 2.90
Seq Identity: 0.32 | Length: 251 / 465
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000116750 | ubiquitin C-terminal hydrolase L5 |
Protein Length: 465 | Molecular Weight (kDa): 54.46
UniProt ID(s): Q8IIJ6
PDB ID(s): None
Isoelectric Point: 5.36
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001578 | Peptidase C12, ubiquitin carboxyl-terminal hydrolase |
| InterPro | IPR038765 | Papain-like cysteine peptidase superfamily |
| PFam | PF01088 | Peptidase C12, ubiquitin carboxyl-terminal hydrolase |
| Superfamily | SSF54001 | Papain-like cysteine peptidase superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 34 | 10 | 33 |
| disruptive | 18 | 55 | 24 | 63 |
| missense | 6 | 35 | 22 | 40 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 21 | 53 | 16 | 29 |
| disruptive | 34 | 100 | 48 | 103 |
| missense | 13 | 75 | 32 | 67 |
PlasmoDB Total SNPs: 123
Non-coding: 69 | Synonymous: 39 | Nonsynonymous: 15 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16925553 | Identification by functional proteomics of a deubiquitinating/deNeddylatingenzyme in Plasmodium falciparum. | Artavanis-Tsakonas K, Misaghi S, ..., Ploegh HL | 10.1111/j.1365-2958.2006.05307.x |
| 31658303 | Nedd8 hydrolysis by UCH proteases in Plasmodium parasites. | Karpiyevich M, Adjalley S, ..., Artavanis-Tsakonas K | 10.1371/journal.ppat.1008086 |