Genome location: Pf3D7_11_v3:666,027..667,977(-)
Genome classification: Core
Product Description: tyrosine--tRNA ligase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.1 (Tyrosine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005829 | cytosol |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0005524 | ATP binding |
| Function | GO:0004831 | tyrosine-tRNA ligase activity |
| Process | GO:0043039 | tRNA aminoacylation |
| Process | GO:0006437 | tyrosyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.25 | 0.88 |
| Trophozoite | possibly expressed | 1.02 | 0.48 |
| Schizont | possibly expressed | 0.33 | 0.79 |
| Gametocyte | not expressed | 0.32 | 0.83 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1117500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -3.112 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIJ2
"Best" AlphaFill ligand hit: YSA (tyrosyladenylate, Local RMSD=0.70) with 6I5Y (Global RMSD=5.92)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.1 | tyrosine-tRNA ligase | AMPPCMBsulfatefisetintRNATyrchrysinchlorideacacetintyrosinoldiphosphateresveratrolkaempferide... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P0AGJ9 | Tyrosine--tRNA ligase | OrthoMCL | CHEMBL4208126CHEMBL1163085CHEMBL4518549CHEMBL4460886... |
Ortholog Group (OrthoMCL): OG6_101423
Most Similar Human Ortholog: H0YHS6
TM-align score: 0.89 | RMSD: 2.17
Seq Identity: 0.28 | Length: 276 / 561
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000139131 | tyrosyl-tRNA synthetase 2 |
Protein Length: 561 | Molecular Weight (kDa): 66.419
UniProt ID(s): Q8IIJ2
PDB ID(s): None
Isoelectric Point: 9.28
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002305 | Aminoacyl-tRNA synthetase, class Ic |
| InterPro | IPR002307 | Tyrosine-tRNA ligase |
| InterPro | IPR024088 | Tyrosine-tRNA ligase, bacterial-type |
| PFam | PF00579 | Aminoacyl-tRNA synthetase, class Ic |
| Superfamily | SSF52374 | N/A |
| Superfamily | SSF55174 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 20 | 18 | 39 |
| disruptive | 11 | 48 | 26 | 83 |
| missense | 7 | 39 | 24 | 73 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 52 | 29 | 34 |
| disruptive | 26 | 109 | 50 | 120 |
| missense | 17 | 86 | 41 | 88 |
PlasmoDB Total SNPs: 42
Non-coding: 4 | Synonymous: 27 | Nonsynonymous: 11 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 20042123 | A genomic glimpse of aminoacyl-tRNA synthetases in malaria parasite Plasmodiumfalciparum. | Bhatt TK, Kapil C, ..., Sharma A | 10.1186/1471-2164-10-644 |
| 22068597 | Malaria parasite tyrosyl-tRNA synthetase secretion triggers pro-inflammatoryresponses. | Bhatt TK, Khan S, ..., Sharma A | 10.1038/ncomms1522 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 30592748 | Plasmodium apicoplast tyrosyl-tRNA synthetase recognizes an unusual, simplifiedidentity set in cognate tRNATyr. | Cela M, Paulus C, ..., Rudinger-Thirion J | 10.1371/journal.pone.0209805 |