PF3D7_1117700 (RAN)

Genome location: Pf3D7_11_v3:670,173..673,050(-)

Genome classification: Core

Function and Localization

Product Description: GTP-binding nuclear protein RAN/TC4

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:1903561	extracellular vesicle
Component	GO:0020020	food vacuole
Component	GO:0005634	nucleus
Function	GO:0005525	GTP binding
Function	GO:0003924	GTPase activity
Process	GO:0006405	RNA export from nucleus
Process	GO:0006886	intracellular protein transport
Process	GO:0006606	protein import into nucleus
Process	GO:0051726	regulation of cell cycle
Process	GO:0000054	ribosomal subunit export from nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.66	0.34
Trophozoite	expressed	2.77	0.06
Schizont	expressed	1.42	0.37
Gametocyte	expressed	1.47	0.34

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1117700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.492 | MFS: 0 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0930300)

Relative Growth Rate: 0.20 ± 0.20
Confidence: 4.60

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q7KQK6

"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=0.18) with 3LAW (Global RMSD=4.80)

No associated EC numbers

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P32836	GTP-binding nuclear protein GSP2/CNR2		HOGENOM, OMA, OrthoDB, OrthoMCL	CAS_10186399CHEMBL376700(+/-)-SLV319[3H]Rimonabant
P32835	GTP-binding nuclear protein GSP1/CNR1		HOGENOM, OrthoDB, OrthoMCL	CAS_10186399CAS_9810861CAS_5311257CHEMBL50925...

Orthology Information

Ortholog Group (OrthoMCL): OG6_101761

Most Similar Human Ortholog: F5H018

TM-align score: 0.96 | RMSD: 1.09

Seq Identity: 0.70 | Length: 195 / 214

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000132341	RAN, member RAS oncogene family

Protein Information

Protein Length: 214 | Molecular Weight (kDa): 24.875

UniProt ID(s): P38545, Q7KQK6

PDB ID(s): None

Isoelectric Point: 7.87

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001806	Small GTPase
InterPro	IPR002041	Ran GTPase
InterPro	IPR005225	Small GTP-binding protein domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00071	Small GTPase
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	0	0	0
disruptive	0	0	0	0
missense	0	0	0	0

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	0	0	0	0
disruptive	0	0	0	1
missense	0	0	0	0

PlasmoDB Total SNPs: 118

Non-coding: 111 | Synonymous: 6 | Nonsynonymous: 0 | Stop Codon: 1

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
21136929	Food vacuole proteome of the malarial parasite Plasmodium falciparum.	Lamarque M, Tastet C, ..., Dubremetz JF	10.1002/prca.200700112
28944300	Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome.	Abdi A, Yu L, ..., Rayner J	10.12688/wellcomeopenres.11910.2
30379851	Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation.	Briquet S, Ourimi A, ..., Vaquero C	10.1371/journal.pone.0205596