Product Description: DNA mismatch repair protein MLH
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0032389 | MutLalpha complex |
| Component | GO:0005712 | chiasma |
| Component | GO:0000781 | chromosome, telomeric region |
| Component | GO:0032300 | mismatch repair complex |
| Function | GO:0005524 | ATP binding |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0004519 | endonuclease activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0006298 | mismatch repair |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.20 | 0.90 |
| Trophozoite | expressed | 0.50 | 0.71 |
| Schizont | expressed | 0.33 | 0.78 |
| Gametocyte | expressed | 0.48 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1117800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.154 | MFS: -2.822 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0930200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIJ0
"Best" AlphaFill ligand hit: ACP (adenosine-5'-[beta, gamma-methylene]triphosphate, Local RMSD=0.49) with 6LZJ (Global RMSD=6.15)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101011
Most Similar Human Ortholog: H0Y5L7
TM-align score: 0.94 | RMSD: 1.32
Seq Identity: 0.37 | Length: 142 / 1016
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000076242 | mutL homolog 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 48 | 25 | 56 |
| disruptive | 17 | 78 | 44 | 118 |
| missense | 10 | 69 | 39 | 100 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 85 | 33 | 63 |
| disruptive | 30 | 164 | 70 | 189 |
| missense | 19 | 132 | 63 | 151 |
PlasmoDB Total SNPs: 117
Non-coding: 36 | Synonymous: 51 | Nonsynonymous: 30 | Stop Codon: 0
Protein Length: 1016 | Molecular Weight (kDa): 118.307
PDB ID(s): None
Isoelectric Point: 6.39
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR013507 | DNA mismatch repair protein, S5 domain 2-like |
| InterPro | IPR014762 | DNA mismatch repair, conserved site |
| InterPro | IPR020568 | Ribosomal protein S5 domain 2-type fold |
| InterPro | IPR032189 | DNA mismatch repair protein Mlh1, C-terminal |
| InterPro | IPR036890 | Histidine kinase/HSP90-like ATPase superfamily |
| InterPro | IPR038973 | DNA mismatch repair protein MutL/Mlh/Pms |
| PFam | PF01119 | DNA mismatch repair protein, S5 domain 2-like |
| PFam | PF13589 | DNA mismatch repair protein Mlh1, C-terminal |
| PFam | PF16413 | |
| Superfamily | SSF54211 | Ribosomal protein S5 domain 2-type fold |
| Superfamily | SSF55874 | Histidine kinase/HSP90-like ATPase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 22079692 | Plasmodium falciparum MLH is schizont stage specific endonuclease. | Tarique M, Satsangi AT, ..., Tuteja R | 10.1016/j.molbiopara.2011.10.012 |
| 23185322 | Plasmodium falciparum UvrD helicase translocates in 3' to 5' direction,colocalizes with MLH and modulates its activity through physical interaction. | Ahmad M, Ansari A, ..., Tuteja R | 10.1371/journal.pone.0049385 |
| 28376558 | Pf AP2Tel, harbouring a non-canonical D NA-binding A P2 domain, binds to Plasmodiumfalciparum telomeres | Sierra-Miranda M, Vembar SS, ..., Hernandez-Rivas R | 10.1111/cmi.12742 |