PF3D7_1118300

Genome location: Pf3D7_11_v3:693,215..699,458(+)

Genome classification: Core

Function and Localization

Product Description: insulinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.24.56 (Insulysin)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0004222	metalloendopeptidase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.46	0.41
Trophozoite	expressed	0.97	0.50
Schizont	expressed	0.51	0.71
Gametocyte	expressed	0.80	0.57

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1118300

Resistome Missense Mutations: E863D

Resistome Compounds with Missense Mutations: Cipargamin/KAE609/NITD609

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.04 | #Insertions: 4

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0929800)

Relative Growth Rate: 1.09 ± 0.18
Confidence: 4.80

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0929800)

Modification: Disrupted | RMgm-2621

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8III5

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.24.56	insulysin	ATPNEMDTTEDTAPCMBEGTAPMSFInsL3amylinnestinUb1-72Ub1-74...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105738

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	31	60	37	78
disruptive	62	114	72	165
missense	35	82	59	127

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	46	105	61	90
disruptive	101	228	112	240
missense	51	176	87	151

PlasmoDB Total SNPs: 339

Non-coding: 140 | Synonymous: 111 | Nonsynonymous: 88 | Stop Codon: 0

Protein Information

Protein Length: 1488 | Molecular Weight (kDa): 173.625

UniProt ID(s): Q8III5

PDB ID(s): None

Isoelectric Point: 5.06

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007863	Peptidase M16, C-terminal
InterPro	IPR011249	Metalloenzyme, LuxS/M16 peptidase-like
InterPro	IPR011765	Peptidase M16, N-terminal
PFam	PF00675	Peptidase M16, N-terminal
PFam	PF05193	Peptidase M16, C-terminal
Superfamily	SSF63411	Metalloenzyme, LuxS/M16 peptidase-like

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104