About Gene List

PF3D7_1118400 (HAD4)

Genome location: Pf3D7_11_v3:699,508..702,063(-)

Genome classification: Core

Function and Localization

Product Description: haloacid dehalogenase-like hydrolase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Function GO:0016791 phosphatase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.06 0.97
Trophozoite not expressed 0.17 0.89
Schizont not expressed 0.55 0.69
Gametocyte not expressed 0.28 0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1118400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.992 | MFS: -2.562 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0929700)

  • Relative Growth Rate: 0.99 ± 0.05
  • Confidence: 7.27

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0929700)

Modification: Disrupted | RMgm-2620

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8III4

"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.02) with 4YGR (Global RMSD=2.87)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_119263

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 213

Non-coding: 198 | Synonymous: 12 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 306 | Molecular Weight (kDa): 36.489

UniProt ID(s): Q8III4

PDB ID(s): None

Isoelectric Point: 6.26

Protein Domain Annotations:

Source Family ID Description
InterPro IPR006439 HAD hydrolase, subfamily IA
InterPro IPR036412 HAD-like superfamily
InterPro IPR041492 Haloacid dehalogenase-like hydrolase
PFam PF13419 Haloacid dehalogenase-like hydrolase
Superfamily SSF56784 HAD-like superfamily

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
33821563 Non-canonical metabolic pathways in the malaria parasite detected byisotope-tracing metabolomics. Cobbold SA, V Tutor M, ..., McConville MJ 10.15252/msb.202010023