PF3D7_1118500 (NOP56)

Genome location: Pf3D7_11_v3:702,276..705,399(+)

Genome classification: Core

Function and Localization

Product Description: nucleolar protein 56, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0031428	box C/D RNP complex
Component	GO:0005634	nucleus
Component	GO:0032040	small-subunit processome
Function	GO:0030515	snoRNA binding
Process	GO:0006364	rRNA processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.46	0.77
Trophozoite	expressed	0.30	0.83
Schizont	expressed	0.17	0.88
Gametocyte	expressed	0.37	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1118500

Resistome Missense Mutations: D481E, H545Y

Resistome Compounds with Missense Mutations: AN13762, GNF-Pf-3600

Resistome # Samples with Disruptive Mutations: 3 (3 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.123 | MFS: -2.956 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0929600)

Relative Growth Rate: 0.20 ± 0.63
Confidence: 2.32

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8III3

"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.97) with 3NMU (Global RMSD=5.37)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101480

Most Similar Human Ortholog: A0A494C128

TM-align score: 0.89 | RMSD: 1.50

Seq Identity: 0.38 | Length: 439 / 594

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000101361	NOP56 ribonucleoprotein

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	44	15	34
disruptive	21	44	26	70
missense	16	39	22	51

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	72	28	26
disruptive	34	116	61	154
missense	20	81	42	94

PlasmoDB Total SNPs: 173

Non-coding: 106 | Synonymous: 36 | Nonsynonymous: 31 | Stop Codon: 0

Protein Information

Protein Length: 594 | Molecular Weight (kDa): 69.39

UniProt ID(s): Q8III3

PDB ID(s): None

Isoelectric Point: 5.7

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002687	Nop domain
InterPro	IPR012974	NOP5, N-terminal
InterPro	IPR012976	NOSIC
InterPro	IPR036070	Nop domain superfamily
PFam	PF01798	Nop domain
PFam	PF08156	NOP5, N-terminal
Superfamily	SSF89124	Nop domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
15477199	Myosin-like sequences in the malaria parasite Plasmodium falciparum bind humanerythrocyte membrane protein 4.1.	Lanzillotti R, Coetzer TL	https://pubmed.ncbi.nlm.nih.gov/15477199/
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
30379851	Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation.	Briquet S, Ourimi A, ..., Vaquero C	10.1371/journal.pone.0205596