About Gene List

PF3D7_1119100

Genome location: Pf3D7_11_v3:720,555..722,354(+)

Genome classification: Core

Function and Localization

Product Description: tRNA m(1)G methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.221 (tRNA (guanine(9)-N(1))-methyltransferase);2.1.1.31 (Transferred entry: 2.1.1.221 and 2.1.1.228)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005634 nucleus
Function GO:0009019 tRNA (guanine-N1-)-methyltransferase activity
Function GO:0000049 tRNA binding
Process GO:0090646 mitochondrial tRNA processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 0.35 0.84
Trophozoite expressed 0.87 0.55
Schizont expressed 0.18 0.89
Gametocyte expressed 0.76 0.59

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1119100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.404 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0929000)

  • Relative Growth Rate: 0.98 ± 0.09
  • Confidence: 6.19

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0929000)

Modification: Disrupted | RMgm-2614

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIH6

"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=1.02) with 4FMW (Global RMSD=1.99)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.1.1.221 tRNA (guanine9-N1)-methyltransferase No BRENDA inhibitors
2.1.1.31 tRNA (guanine-N1-)-methyltransferase No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102182

Most Similar Human Ortholog: X6REK4

TM-align score: 0.62 | RMSD: 3.39

Seq Identity: 0.23 | Length: 159 / 324

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000145331 tRNA methyltransferase 10A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 1 19 8 22
disruptive 2 25 21 49
missense 2 24 21 38

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 2 36 13 26
disruptive 18 84 39 94
missense 7 66 31 54

PlasmoDB Total SNPs: 81

Non-coding: 66 | Synonymous: 13 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 324 | Molecular Weight (kDa): 38.793

UniProt ID(s): Q8IIH6

PDB ID(s): None

Isoelectric Point: 9.95

Protein Domain Annotations:

Source Family ID Description
InterPro IPR007356 tRNA (guanine-N1-)-methyltransferase, eukaryotic
InterPro IPR016009 tRNA methyltransferase TRMD/TRM10-type domain
InterPro IPR016653 tRNA (guanine(9)-N(1))-methyltransferase TRM10/TRM10A
PFam PF01746 tRNA methyltransferase TRMD/TRM10-type domain
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104