Product Description: ubiquitin-protein ligase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: 6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0061631 | ubiquitin conjugating enzyme activity |
| Process | GO:0000209 | protein polyubiquitination |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.49 | 0.76 |
| Trophozoite | expressed | 0.70 | 0.62 |
| Schizont | expressed | 0.61 | 0.65 |
| Gametocyte | expressed | 0.59 | 0.67 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1119400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.279 | #Insertions: 6
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0928700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0928700)
Modification: Disrupted | RMgm-2611
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIH3
"Best" AlphaFill ligand hit: AYE (allylamine, Local RMSD=0.34) with 6XZ1 (Global RMSD=3.37)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.2.19 | ubiquitin-protein ligase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100865
Most Similar Human Ortholog: H7C0Y1
TM-align score: 0.60 | RMSD: 2.49
Seq Identity: 0.00 | Length: 49 / 2147
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000009335 | ubiquitin protein ligase E3C |
| ENSG00000151148 | ubiquitin protein ligase E3B |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 30 | 100 | 29 | 104 |
| disruptive | 79 | 268 | 159 | 322 |
| missense | 45 | 209 | 136 | 258 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 54 | 168 | 78 | 138 |
| disruptive | 168 | 632 | 283 | 625 |
| missense | 91 | 481 | 217 | 348 |
PlasmoDB Total SNPs: 428
Non-coding: 166 | Synonymous: 144 | Nonsynonymous: 118 | Stop Codon: 0
Protein Length: 2147 | Molecular Weight (kDa): 256.822
UniProt ID(s): Q8IIH3
PDB ID(s): None
Isoelectric Point: 7.53
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000569 | HECT domain |
| InterPro | IPR035983 | HECT, E3 ligase catalytic domain |
| PFam | PF00632 | HECT domain |
| Superfamily | SSF56204 | HECT, E3 ligase catalytic domain |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368868 | Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14. | Gardner MJ, Shallom SJ, ..., Fraser CM | 10.1038/nature01094 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |