Product Description: alternative splicing factor ASF-1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003729 | mRNA binding |
| Process | GO:0000380 | alternative mRNA splicing, via spliceosome |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.43 | 0.79 |
| Trophozoite | expressed | 0.53 | 0.69 |
| Schizont | possibly expressed | 0.25 | 0.83 |
| Gametocyte | not expressed | 0.36 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1119800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -2.88 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIG9
"Best" AlphaFill ligand hit: 1PG (2-(2-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethanol, Local RMSD=1.77) with 5W0G (Global RMSD=6.02)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 4 | 10 | 8 |
| disruptive | 0 | 7 | 2 | 10 |
| missense | 0 | 7 | 2 | 10 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 19 | 8 | 7 |
| disruptive | 3 | 21 | 12 | 32 |
| missense | 2 | 13 | 9 | 22 |
PlasmoDB Total SNPs: 41
Non-coding: 32 | Synonymous: 8 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 200 | Molecular Weight (kDa): 23.652
UniProt ID(s): Q8IIG9
PDB ID(s): None
Isoelectric Point: 9.96
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000504 | RNA recognition motif domain |
| InterPro | IPR035979 | RNA-binding domain superfamily |
| PFam | PF00076 | RNA recognition motif domain |
| Superfamily | SSF54928 | RNA-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 27381095 | The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. | Bunnik EM, Batugedara G, ..., Le Roch KG | 10.1186/s13059-016-1014-0 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 38407959 | A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae reveals novel insights into factors that stabilize chromatin structure on newly replicated DNA | Gao E, Brown JAR, Jung S, Howe LJ | 10.1093/genetics/iyae016 |
| 36914923 | The patterns and participants of parental histone recycling during DNAreplication in Saccharomyces cerevisiae | Shan Z, Zhang Y, ..., Zhu B | 10.1007/s11427-022-2267-6 |
| 38164276 | Carcinogenic Role and Clinical Significance of Histone H3-H4 ChaperoneAnti-silencing Function 1 B (ASF1B) in Lung Adenocarcinoma | Song C, Song Y, ..., Geng Q | 10.7150/jca.88777 |
| 38262410 | Regulation of replicative histone RNA metabolism by the histone chaperone ASF1 | Mendiratta S, Ray-Gallet D, ..., Almouzni G | 10.1016/j.molcel.2023.12.038 |
| 38112417 | Histone binding of ASF1 is required for fruiting body development but not forgenome stability in the filamentous fungus Sordaria macrospora | Breuer J, Busche T, Kalinowski J, Nowrousian M | 10.1128/mbio.02896-23 |
| 38261383 | Functional analysis of chromatin-associated proteins in Sordaria macrosporareveals similar roles for RTT109 and ASF1 in development and DNA damage response | Breuer J, Ferreira DEA, ..., Nowrousian M | 10.1093/g3journal/jkae019 |