About Gene List

PF3D7_1120500

Genome location: Pf3D7_11_v3:771,490..775,327(+)

Genome classification: Core

Function and Localization

Product Description: tRNA nucleotidyltransferase, putative

SignalP Peptide: MNIFGLLLLNLLINVIFI

# Transmembrane Domains: 0

EC Numbers: 2.7.7.72 (CCA tRNA nucleotidyltransferase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast
Function GO:0052929 ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity
Function GO:0052928 CTP:3'-cytidine-tRNA cytidylyltransferase activity
Function GO:0016779 nucleotidyltransferase activity
Process GO:0001680 tRNA 3'-terminal CCA addition

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 0.78 0.63
Trophozoite expressed 0.99 0.49
Schizont possibly expressed 0.23 0.85
Gametocyte not expressed 0.73 0.60

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1120500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.169 | MFS: -3.259 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0927800)

  • Relative Growth Rate: 0.23 ± 0.24
  • Confidence: 4.24

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIG2

"Best" AlphaFill ligand hit: 2TM (cmpcpp, Local RMSD=0.33) with 6Q52 (Global RMSD=2.29)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.7.7.72 CCA tRNA nucleotidyltransferase Neocuproine2,2-dipyridylethylnitrosourea2,2,2-Terpyridyl1,7-phenanthroline1,10-phenanthrolinebathophenanthroline

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104034

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 6 23 13 40
disruptive 10 54 36 123
missense 8 52 36 110

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 9 50 19 43
disruptive 22 123 90 154
missense 18 107 82 117

PlasmoDB Total SNPs: 108

Non-coding: 76 | Synonymous: 20 | Nonsynonymous: 12 | Stop Codon: 0

Protein Information

Protein Length: 791 | Molecular Weight (kDa): 94.405

UniProt ID(s): Q8IIG2

PDB ID(s): None

Isoelectric Point: 9.34

Protein Domain Annotations:

Source Family ID Description
InterPro IPR002646 Poly A polymerase, head domain
InterPro IPR043519 Nucleotidyltransferase superfamily
PFam PF01743 Poly A polymerase, head domain
Superfamily SSF81301 Nucleotidyltransferase superfamily
Superfamily SSF81891

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
30212465 Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. Boucher MJ, Ghosh S, ..., Yeh E 10.1371/journal.pbio.2005895