PF3D7_1121000 (DHHC3)

Genome location: Pf3D7_11_v3:792,188..794,151(+)

Genome classification: Core

Function and Localization

Product Description: palmitoyltransferase DHHC3

SignalP Peptide: N/A

# Transmembrane Domains: 4

EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.225 (Protein S-acyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005794	Golgi apparatus
Component	GO:0005783	endoplasmic reticulum
Function	GO:0016409	palmitoyltransferase activity
Function	GO:0019706	protein-cysteine S-palmitoyltransferase activity
Process	GO:0018230	peptidyl-L-cysteine S-palmitoylation
Process	GO:0018345	protein palmitoylation
Process	GO:0006612	protein targeting to membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	expressed	0.20	0.87
Schizont	expressed	0.27	0.82
Gametocyte	expressed	0.77	0.61

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1121000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.795 | MFS: -2.768 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0927300)

Relative Growth Rate: 0.25 ± 0.17
Confidence: 4.93

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0927300)

Modification: Tagged | RMgm-897

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIF7

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.1.-	Ex: [myelin-proteolipid] O-palmitoyltransferase	CoAKClArgATPADPEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4...
2.3.1.225	protein S-acyltransferase	stearoyl-CoAmyristoyl-CoA2-Bromopalmitate2-bromo-palmitateacyl-CoA binding protein

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102853

Most Similar Human Ortholog: D6RE92

TM-align score: 0.76 | RMSD: 1.22

Seq Identity: 0.07 | Length: 41 / 293

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000159714	zinc finger DHHC-type containing 1
ENSG00000188818	zinc finger DHHC-type containing 11
ENSG00000206077	zinc finger DHHC-type containing 11B

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	10	4	18
disruptive	1	5	1	27
missense	1	5	1	23

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	18	9	18
disruptive	3	34	17	63
missense	1	19	13	42

PlasmoDB Total SNPs: 51

Non-coding: 39 | Synonymous: 12 | Nonsynonymous: 0 | Stop Codon: 0

Protein Information

Protein Length: 293 | Molecular Weight (kDa): 34.78

UniProt ID(s): Q8IIF7

PDB ID(s): None

Isoelectric Point: 8

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001594	Palmitoyltransferase, DHHC domain
PFam	PF01529	Palmitoyltransferase, DHHC domain
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
27060339	Study of Plasmodium falciparum DHHC palmitoyl transferases identifies a role forPfDHHC9 in gametocytogenesis.	Tay CL, Jones ML, ..., Rayner JC	10.1111/cmi.12599