Product Description: protein GCN20
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0016020 | membrane |
| Function | GO:0005524 | ATP binding |
| Function | GO:0042626 | ATPase-coupled transmembrane transporter activity |
| Process | GO:0006417 | regulation of translation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.85 | 0.61 |
| Trophozoite | expressed | 0.90 | 0.53 |
| Schizont | expressed | 0.19 | 0.89 |
| Gametocyte | expressed | 0.64 | 0.65 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1121700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.396 | #Insertions: 5
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0926600)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_0928600)
Modification: Disrupted | RMgm-4779
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIE9
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.01) with 6Z63 (Global RMSD=4.59)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100624
Most Similar Human Ortholog: Q9NUQ8
TM-align score: 0.73 | RMSD: 2.75
Seq Identity: 0.37 | Length: 552 / 815
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000161204 | ATP binding cassette subfamily F member 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 23 | 18 | 30 |
| disruptive | 10 | 27 | 28 | 67 |
| missense | 10 | 25 | 23 | 57 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 57 | 18 | 35 |
| disruptive | 22 | 85 | 47 | 124 |
| missense | 15 | 68 | 36 | 86 |
PlasmoDB Total SNPs: 137
Non-coding: 105 | Synonymous: 19 | Nonsynonymous: 13 | Stop Codon: 0
Protein Length: 815 | Molecular Weight (kDa): 95.375
PDB ID(s): None
Isoelectric Point: 6.98
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003439 | ABC transporter-like |
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR017871 | ABC transporter, conserved site |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR032781 | ABC-transporter extension domain |
| PFam | PF00005 | ABC transporter-like |
| PFam | PF12848 | ABC-transporter extension domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 11512154 | The ABC transporter genes of Plasmodium falciparum and drug resistance | Peel SA | 10.1054/drup.2001.0183 |
| 35601823 | Enhancement and mapping of tolerance to salt stress and 5-fluorocytosine insynthetic yeast strains via SCRaMbLE | Kang J, Li J, ..., Yuan Y | 10.1016/j.synbio.2022.04.003 |
| 31937829 | Global translational repression induced by iron deficiency in yeast depends onthe Gcn2/eIF2alpha pathway | Romero AM, Ramos-Alonso L, ..., Martinez-Pastor MT | 10.1038/s41598-019-57132-0 |
| 29499090 | Arabidopsis nonresponding to oxylipins locus NOXY7 encodes a yeast GCN1 homologthat mediates noncanonical translation regulation and stress adaptation | Izquierdo Y, Kulasekaran S, ..., Castresana C | 10.1111/pce.13182 |
| 37043534 | Differential requirements for P stalk components in activating yeast proteinkinase Gcn2 by stalled ribosomes during stress | Gupta R, Hinnebusch AG | 10.1073/pnas.2300521120 |
| 34900236 | Ribo-Seq and RNA-Seq of TMA46 ( DFRP1) and GIR2 ( DFRP2) knockout yeast strains | Egorov AA, Makeeva DS, ..., Dmitriev SE | 10.12688/f1000research.74727.1 |