PF3D7_1121800

Genome location: Pf3D7_11_v3:823,239..829,313(-)

Genome classification: Core

Function and Localization

Product Description: peptidase M16, putative

SignalP Peptide: MKHTKITKYLTINFFILLTLVFQKYSSC

# Transmembrane Domains: 0

EC Numbers: 3.4.24.55 (Pitrilysin)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Process	GO:0043171	peptide catabolic process
Process	GO:0051603	proteolysis involved in cellular protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	not expressed	0.02	0.99
Schizont	not expressed	0.11	0.94
Gametocyte	possibly expressed	0.44	0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1121800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.86 | #Insertions: 19

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIE8

"Best" AlphaFill ligand hit: FUM (fumaric acid, Local RMSD=0.12) with 4LTE (Global RMSD=3.23)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.24.55	pitrilysin	DTTEDTAPCMBEGTAZincovcysteineantipainbacitracinchymostatinphosphoramidonN-ethylmaleimideChelating agents...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_108964

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	49	32	96
disruptive	26	158	78	204
missense	21	148	73	191

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	13	101	60	112
disruptive	52	321	124	301
missense	38	275	104	272

PlasmoDB Total SNPs: 121

Non-coding: 0 | Synonymous: 83 | Nonsynonymous: 38 | Stop Codon: 0

Protein Information

Protein Length: 2024 | Molecular Weight (kDa): 238.898

UniProt ID(s): Q8IIE8

PDB ID(s): None

Isoelectric Point: 6.96

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR011249	Metalloenzyme, LuxS/M16 peptidase-like
InterPro	IPR011765	Peptidase M16, N-terminal
PFam	PF00675	Peptidase M16, N-terminal
Superfamily	SSF63411	Metalloenzyme, LuxS/M16 peptidase-like