PF3D7_1122800 (CDPK6)

Genome location: Pf3D7_11_v3:871,050..877,707(+)

Genome classification: Core

Function and Localization

Product Description: calcium-dependent protein kinase 6

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.11.13 (Protein kinase C);2.7.11.17 (Calcium/calmodulin-dependent protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:1903561	extracellular vesicle
Component	GO:0005634	nucleus
Function	GO:0009931	calcium-dependent protein serine/threonine kinase activity
Function	GO:0005516	calmodulin binding
Process	GO:0035556	intracellular signal transduction
Process	GO:0018105	peptidyl-serine phosphorylation
Process	GO:0046777	protein autophosphorylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.48	0.79
Trophozoite	expressed	0.30	0.82
Schizont	expressed	0.45	0.74
Gametocyte	expressed	0.41	0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1122800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.818 | #Insertions: 6

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0925500)

Relative Growth Rate: 0.81 ± 0.07
Confidence: 6.85

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0925500)

Modification: Disrupted | RMgm-4548

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IID5

"Best" AlphaFill ligand hit: D1D ((4s,5s)-1,2-dithiane-4,5-diol, Local RMSD=0.14) with 2YA9 (Global RMSD=2.04)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.13	protein kinase C	BRENDA SOAP query unsuccessful
2.7.11.17	Ca2+/calmodulin-dependent protein kinase	NOADPGTPW13AIPEDTAEGTADTNBKN93KN62KN-93KN-62...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_136893

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	14	62	42	86
disruptive	38	132	67	231
missense	19	108	57	197

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	21	143	51	83
disruptive	74	280	174	341
missense	41	221	144	218

PlasmoDB Total SNPs: 243

Non-coding: 131 | Synonymous: 77 | Nonsynonymous: 34 | Stop Codon: 1

Protein Information

Protein Length: 1617 | Molecular Weight (kDa): 191.814

UniProt ID(s): Q8IID5

PDB ID(s): 3MSE

Isoelectric Point: 5.17

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR002048	EF-hand domain
InterPro	IPR008271	Serine/threonine-protein kinase, active site
InterPro	IPR011009	Protein kinase-like domain superfamily
InterPro	IPR011992	EF-hand domain pair
InterPro	IPR018247	EF-Hand 1, calcium-binding site
PFam	PF00069	Protein kinase domain
PFam	PF13202	EF-hand domain
Superfamily	SSF47473	EF-hand domain pair
Superfamily	SSF56112	Protein kinase-like domain superfamily

Associated Publications

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
33608523	20S proteasomes secreted by the malaria parasite promote its growth.	Dekel E, Yaffe D, ..., Regev-Rudzki N	10.1038/s41467-021-21344-8
26499803	Evaluation of the basic functions of six calcium-dependent protein kinases inToxoplasma gondii using CRISPR-Cas9 system	Wang JL, Huang SY, ..., Zhu XQ	10.1007/s00436-015-4791-6
26755159	Analysis of Noncanonical Calcium-Dependent Protein Kinases in Toxoplasma gondiiby Targeted Gene Deletion Using CRISPR/Cas9	Long S, Wang Q, Sibley LD	10.1128/IAI.01173-15
27923926	Reduced Activity of Mutant Calcium-Dependent Protein Kinase 1 Is Compensated inPlasmodium falciparum through the Action of Protein Kinase G	Bansal A, Ojo KK, ..., Miller LH	10.1128/mBio.02011-16
25526188	Identification and bioinformatic analysis of a putative calcium-dependent proteinkinase (CDPK6) from Toxoplasma gondii	Zhang NZ, Huang SY, ..., Zhu XQ	10.4238/2014.December.18.9