Product Description: calcium-dependent protein kinase 7
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.17 (Calcium/calmodulin-dependent protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0048471 | perinuclear region of cytoplasm |
| Component | GO:0031982 | vesicle |
| Function | GO:0005546 | phosphatidylinositol-4,5-bisphosphate binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0004672 | protein kinase activity |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0006656 | phosphatidylcholine biosynthetic process |
| Process | GO:0043065 | positive regulation of apoptotic process |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 1.62 | 0.37 |
| Trophozoite | expressed | 1.35 | 0.38 |
| Schizont | expressed | 2.07 | 0.21 |
| Gametocyte | possibly expressed | 2.04 | 0.16 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1123100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.214 | MFS: -2.689 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0925200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IID2
"Best" AlphaFill ligand hit: PGO (s-1,2-propanediol, Local RMSD=0.03) with 2VZ6 (Global RMSD=4.98)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.17 | Ca2+/calmodulin-dependent protein kinase | NOADPGTPW13AIPEDTAEGTADTNBKN93KN62KN-93KN-62... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O94806 | Serine/threonine-protein kinase D3 | Homo sapiens | OrthoMCL BLAST | MLS000043346kb-NB142-70kb-NB165-09kb-NB165-31... |
Protein Length: 2265 | Molecular Weight (kDa): 265.136
PDB ID(s): None
Isoelectric Point: 5.79
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR001849 | Pleckstrin homology domain |
| InterPro | IPR002048 | EF-hand domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR011992 | EF-hand domain pair |
| InterPro | IPR017441 | Protein kinase, ATP binding site |
| InterPro | IPR018247 | EF-Hand 1, calcium-binding site |
| PFam | PF00069 | Protein kinase domain |
| PFam | PF00169 | Pleckstrin homology domain |
| Superfamily | SSF47473 | EF-hand domain pair |
| Superfamily | SSF50729 | Protein kinase-like domain superfamily |
| Superfamily | SSF56112 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 92 | 51 | 118 |
| disruptive | 56 | 164 | 111 | 244 |
| missense | 26 | 128 | 96 | 195 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 35 | 209 | 73 | 123 |
| disruptive | 102 | 384 | 172 | 422 |
| missense | 43 | 281 | 140 | 284 |
PlasmoDB Total SNPs: 323
Non-coding: 119 | Synonymous: 129 | Nonsynonymous: 74 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 24895132 | Regulation of Plasmodium falciparum development by calcium-dependent proteinkinase 7 (PfCDPK7). | Kumar P, Tripathi A, ..., Sharma P | 10.1074/jbc.M114.561670 |
| 30429842 | Characterization of a Protein Phosphatase Type-1 and a Kinase Anchoring Proteinin Plasmodium falciparum. | Lenne A, De Witte C, ..., Pierrot C | 10.3389/fmicb.2018.02617 |
| 34866326 | PI4-kinase and PfCDPK7 signaling regulate phospholipid biosynthesis in Plasmodiumfalciparum. | Maurya R, Tripathi A, ..., Sharma P | 10.15252/embr.202154022 |
| 33635921 | Protein kinase TgCDPK7 regulates vesicular trafficking and phospholipid synthesisin Toxoplasma gondii | Bansal P, Antil N, ..., Sharma P | 10.1371/journal.ppat.1009325 |
| 26755159 | Analysis of Noncanonical Calcium-Dependent Protein Kinases in Toxoplasma gondii by Targeted Gene Deletion Using CRISPR/Cas9 | Long S, Wang Q, Sibley LD | 10.1128/IAI.01173-15 |
| 24011186 | The Toxoplasma gondii calcium-dependent protein kinase 7 is involved in earlysteps of parasite division and is crucial for parasite survival | Morlon-Guyot J, Berry L, ..., Daher W | 10.1111/cmi.12186 |
| 34336254 | Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) ShowedPotential Targets for Toxoplasma gondii Vaccine | Taghipour A, Tavakoli S, ..., Soltani S | 10.1155/2021/9974509 |
| 21318369 | Expression of the rice CDPK-7 in sorghum: molecular and phenotypic analyses | Mall TK, Dweikat I, ..., Clemente T | 10.1007/s11103-011-9741-9 |