PF3D7_1124000 (ERO1)

Genome location: Pf3D7_11_v3:947,614..951,244(+)

Genome classification: Core

Function and Localization

Product Description: endoplasmic reticulum oxidoreductin, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: 5.3.4.1 (Protein disulfide-isomerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020036	Maurer's cleft
Component	GO:0005783	endoplasmic reticulum
Component	GO:0005789	endoplasmic reticulum membrane
Function	GO:0016491	oxidoreductase activity
Function	GO:0003756	protein disulfide isomerase activity
Function	GO:0015035	protein-disulfide reductase activity
Process	GO:0006457	protein folding
Process	GO:0034975	protein folding in endoplasmic reticulum

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.21	0.89
Trophozoite	expressed	0.42	0.74
Schizont	expressed	0.36	0.78
Gametocyte	expressed	1.31	0.39

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1124000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -3.107 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIC3

"Best" AlphaFill ligand hit: NEN (1-ethyl-pyrrolidine-2,5-dione, Local RMSD=1.65) with 1RQ1 (Global RMSD=4.47)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.3.4.1	protein disulfide-isomerase	NEM16F16LOC14AsO43-CCF642MA3 018MA3 019acroleinneomycinpeptidesgenisteinkanamycin...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q96HE7	ERO1-like protein alpha	Homo sapiens	OrthoDB	CHEMBL1668289CHEMBL1668291
Q8R180	ERO1-like protein alpha		OrthoDB	CHEMBL4060344

Orthology Information

Ortholog Group (OrthoMCL): OG6_101008

Most Similar Human Ortholog: G3V2H0

TM-align score: 0.69 | RMSD: 1.86

Seq Identity: 0.37 | Length: 65 / 465

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000086619	endoplasmic reticulum oxidoreductase 1 beta
ENSG00000197930	endoplasmic reticulum oxidoreductase 1 alpha

Protein Information

Protein Length: 465 | Molecular Weight (kDa): 55.046

UniProt ID(s): Q8IIC3

PDB ID(s): None

Isoelectric Point: 8.18

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007266	Endoplasmic reticulum oxidoreductin 1
InterPro	IPR037192	ERO1-like superfamily
PFam	PF04137	Endoplasmic reticulum oxidoreductin 1
Superfamily	SSF110019	ERO1-like superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	17	13	28
disruptive	11	10	2	30
missense	10	9	1	21

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	32	20	30
disruptive	18	24	33	69
missense	12	17	23	42

PlasmoDB Total SNPs: 212

Non-coding: 184 | Synonymous: 20 | Nonsynonymous: 8 | Stop Codon: 0

Associated Publications

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
31631203	A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum.	Das D, Krishnan SR, Roy A, Bulusu G	10.1039/c9mo00124g
37966975	Optimization of Protein Folding for Improved Secretion of Human Serum Albumin Fusion Proteins in Saccharomyces cerevisiae	Li Y, Xiao C, ..., Huang M	10.1021/acs.jafc.3c05330
38007054	Fingerprint of the oxido-reductase ERO1: A protein disulfide bond producer and supporter of cancer	Zito E, Guarrera L, Janssen-Heininger YMW	10.1016/j.bbcan.2023.189027
37652992	Biochemical analysis of Komagataella phaffii oxidative folding proposes novelregulatory mechanisms of disulfide bond formation in yeast	Palma A, Rettenbacher LA, ..., Ruddock L	10.1038/s41598-023-41375-z
37209206	Overexpression of genes by stress-responsive promoters increases proteinsecretion in Saccharomyces cerevisiae	Xiao C, Xue S, ..., Huang M	10.1007/s11274-023-03646-9
37437824	Identification and functional analysis of endoplasmic reticulum oxidoreductase 1(ERO1) from the green mud crab Scylla paramamosain: The first evidence of ERO1involved in invertebrate immune response	Lin CY, Zhang YM, ..., Dong WR	10.1016/j.fsi.2023.108931