Product Description: DNA-directed RNA polymerase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0034245 | mitochondrial DNA-directed RNA polymerase complex |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0003677 | DNA binding |
| Function | GO:0003899 | DNA-directed 5'-3' RNA polymerase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0006390 | mitochondrial transcription |
| Process | GO:0006351 | transcription, DNA-templated |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.25 | 0.88 |
| Trophozoite | expressed | 0.87 | 0.56 |
| Schizont | not expressed | 0.11 | 0.93 |
| Gametocyte | not expressed | 1.22 | 0.40 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1125300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.147 | MFS: -2.841 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIB0
"Best" AlphaFill ligand hit: APC (alpha,beta-methyleneadenosine-5'-triphosphate, Local RMSD=0.19) with 1S0V (Global RMSD=4.67)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101824
Most Similar Human Ortholog: O00411
TM-align score: 0.52 | RMSD: 5.34
Seq Identity: 0.27 | Length: 725 / 1531
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000099821 | RNA polymerase mitochondrial |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 52 | 35 | 73 |
| disruptive | 31 | 127 | 64 | 205 |
| missense | 10 | 98 | 55 | 171 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 25 | 108 | 59 | 89 |
| disruptive | 72 | 256 | 116 | 309 |
| missense | 26 | 194 | 95 | 221 |
PlasmoDB Total SNPs: 171
Non-coding: 12 | Synonymous: 95 | Nonsynonymous: 64 | Stop Codon: 0
Protein Length: 1531 | Molecular Weight (kDa): 179.99
PDB ID(s): None
Isoelectric Point: 9.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002092 | DNA-directed RNA polymerase, phage-type |
| InterPro | IPR029262 | DNA-directed RNA polymerase, N-terminal |
| InterPro | IPR043502 | DNA/RNA polymerase superfamily |
| PFam | PF00940 | DNA-directed RNA polymerase, phage-type |
| PFam | PF14700 | DNA-directed RNA polymerase, N-terminal |
| Superfamily | SSF56672 | DNA/RNA polymerase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 22640832 | Mitochondrial RNA polymerase is an essential enzyme in erythrocytic stages ofPlasmodium falciparum. | Ke H, Morrisey JM, ..., Vaidya AB | 10.1016/j.molbiopara.2012.05.001 |
| 32896572 | PfKsgA1 functions as a transcription initiation factor and interacts with theN-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum. | Gupta A, Shrivastava D, ..., Habib S | 10.1016/j.ijpara.2020.07.010 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 29177737 | Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast | El Hage A, Tollervey D | 10.1007/978-1-4939-7459-7_8 |
| 33278362 | Cryo-EM Structures Reveal Transcription Initiation Steps by Yeast Mitochondrial RNA Polymerase | De Wijngaert B, Sultana S, ..., Das K | 10.1016/j.molcel.2020.11.016 |
| 25183523 | Yeast mitochondrial RNAP conformational changes are regulated by interactions with the mitochondrial transcription factor | Drakulic S, Wang L, ..., Valpuesta JM | 10.1093/nar/gku795 |
| 24096365 | Structure of human mitochondrial RNA polymerase elongation complex | Schwinghammer K, Cheung AC, ..., Cramer P | 10.1038/nsmb.2683 |
| 37460695 | ATFS-1 counteracts mitochondrial DNA damage by promoting repair over transcription | Dai CY, Ng CC, ..., Zuryn S | 10.1038/s41556-023-01192-y |
| 30526856 | Highly efficient 5' capping of mitochondrial RNA with NAD(+) and NADH by yeastand human mitochondrial RNA polymerase | Bird JG, Basu U, ..., Nickels BE | 10.7554/eLife.42179 |