Genome location: Pf3D7_11_v3:996,776..1,000,180(-)
Genome classification: Core
Product Description: mitochondrial import inner membrane translocase subunit TIM44, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005743 | mitochondrial inner membrane |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0051087 | chaperone binding |
| Function | GO:0015450 | protein-transporting ATPase activity |
| Process | GO:0030150 | protein import into mitochondrial matrix |
| Process | GO:0006626 | protein targeting to mitochondrion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.51 | 0.76 |
| Trophozoite | expressed | 0.90 | 0.58 |
| Schizont | expressed | 0.09 | 0.95 |
| Gametocyte | expressed | 0.66 | 0.65 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1125400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -2.949 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0922900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIA9
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102237
Most Similar Human Ortholog: M0QXM9
TM-align score: 0.83 | RMSD: 2.26
Seq Identity: 0.22 | Length: 129 / 470
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000104980 | translocase of inner mitochondrial membrane 44 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 15 | 15 | 26 |
| disruptive | 4 | 14 | 30 | 40 |
| missense | 4 | 14 | 27 | 30 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 45 | 15 | 21 |
| disruptive | 10 | 66 | 45 | 74 |
| missense | 4 | 49 | 35 | 61 |
PlasmoDB Total SNPs: 187
Non-coding: 163 | Synonymous: 16 | Nonsynonymous: 8 | Stop Codon: 0
Protein Length: 470 | Molecular Weight (kDa): 55.81
UniProt ID(s): Q8IIA9
PDB ID(s): None
Isoelectric Point: 9.28
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007379 | Tim44-like domain |
| InterPro | IPR032710 | NTF2-like domain superfamily |
| InterPro | IPR039544 | Tim44-like |
| PFam | PF04280 | Tim44-like domain |
| Superfamily | SSF54427 | NTF2-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 37331598 | Differential sensitivity of the yeast Lon protease Pim1p to impairedmitochondrial respiration | Metzger MB, Scales JL, ..., Weissman AM | 10.1016/j.jbc.2023.104937 |