Genome location: Pf3D7_11_v3:1,018,358..1,022,935(-)
Genome classification: Core
Product Description: autophagy-related protein 7, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0000407 | phagophore assembly site |
| Function | GO:0019778 | Atg12 activating enzyme activity |
| Function | GO:0019779 | Atg8 activating enzyme activity |
| Process | GO:0006501 | C-terminal protein lipidation |
| Process | GO:0000045 | autophagosome assembly |
| Process | GO:0000422 | autophagy of mitochondrion |
| Process | GO:0006995 | cellular response to nitrogen starvation |
| Process | GO:0044805 | late nucleophagy |
| Process | GO:0034727 | piecemeal microautophagy of the nucleus |
| Process | GO:0009657 | plastid organization |
| Process | GO:0032446 | protein modification by small protein conjugation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.11 | 0.94 |
| Trophozoite | expressed | 0.37 | 0.77 |
| Schizont | expressed | 0.11 | 0.92 |
| Gametocyte | possibly expressed | 0.18 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1126100
Resistome Missense Mutations: H674N
Resistome Compounds with Missense Mutations: BI-2536
Resistome # Samples with Disruptive Mutations: 2 (2 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.127 | MFS: -2.95 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIA3
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.52) with 3VH4 (Global RMSD=1.04)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O95352 | Ubiquitin-like modifier-activating enzyme ATG7 | Homo sapiens | OrthoDB | US10865208, Compound I-1US10865208, Compound I-2US10865208, Compound I-3US10865208, Compound I-4... |
Ortholog Group (OrthoMCL): OG6_102679
Most Similar Human Ortholog: H7C2R3
TM-align score: 0.77 | RMSD: 2.21
Seq Identity: 0.25 | Length: 85 / 1316
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000197548 | autophagy related 7 |
Protein Length: 1316 | Molecular Weight (kDa): 156.631
UniProt ID(s): Q8IIA3
PDB ID(s): None
Isoelectric Point: 6.47
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000594 | THIF-type NAD/FAD binding fold |
| InterPro | IPR006285 | Ubiquitin-like modifier-activating enzyme Atg7 |
| InterPro | IPR032197 | Ubiquitin-like modifier-activating enzyme Atg7, N-terminal |
| InterPro | IPR035985 | Ubiquitin-activating enzyme |
| PFam | PF00899 | THIF-type NAD/FAD binding fold |
| PFam | PF16420 | Ubiquitin-like modifier-activating enzyme Atg7, N-terminal |
| Superfamily | SSF69572 | Ubiquitin-activating enzyme |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 40 | 35 | 71 |
| disruptive | 58 | 134 | 56 | 188 |
| missense | 42 | 110 | 47 | 159 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 92 | 53 | 68 |
| disruptive | 88 | 279 | 116 | 304 |
| missense | 58 | 210 | 98 | 205 |
PlasmoDB Total SNPs: 218
Non-coding: 67 | Synonymous: 69 | Nonsynonymous: 80 | Stop Codon: 2
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30726238 | A mutagenesis screen for essential plastid biogenesis genes in human malariaparasites. | Tang Y, Meister TR, ..., Yeh E | 10.1371/journal.pbio.3000136 |
| 38354870 | CDK12 inhibition upregulates ATG7 triggering autophagy via AKT/FOXO3 pathway andenhances anti-PD-1 efficacy in colorectal cancer | Wu Z, Zhang W, ..., Li Q | 10.1016/j.phrs.2024.107097 |
| 38372637 | Role for Caspase-8 in the Release of IL-1beta and Active Caspase-1 from Viable HumanMonocytes during Toxoplasma gondii Infection | Pandori WJ, Matsuno SY, ..., Lodoen MB | 10.4049/jimmunol.2200513 |
| 38326721 | Quercetin Alleviates Lipopolysaccharide-Induced Cardiac Inflammation viaInhibiting Autophagy and Programmed Cell Death | Yu JH, Hu GL, ..., Amin B | 10.3967/bes2024.006 |