PF3D7_1126600

Genome location: Pf3D7_11_v3:1,037,626..1,040,420(-)

Genome classification: Core

Function and Localization

Product Description: steryl ester hydrolase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.1.13 (Sterol esterase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.11	0.94
Trophozoite	possibly expressed	0.29	0.83
Schizont	possibly expressed	0.13	0.92
Gametocyte	possibly expressed	0.60	0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1126600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.059 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0921800)

Modification: Disrupted | RMgm-2584

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8II98

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.1.13	sterol esterase	KClSDSNaFEDTAPMSFMgSO4AS115oleateheparincholateGK03167CD10645...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100408

Most Similar Human Ortholog: Q5VXJ0

TM-align score: 0.78 | RMSD: 3.79

Seq Identity: 0.22 | Length: 361 / 682

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000107798	lipase A, lysosomal acid type
ENSG00000173239	lipase family member M
ENSG00000182333	lipase F, gastric type
ENSG00000204021	lipase family member K

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	11	24	15	40
disruptive	73	128	58	121
missense	28	80	42	78

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	21	48	26	48
disruptive	121	222	107	187
missense	47	138	77	119

PlasmoDB Total SNPs: 168

Non-coding: 52 | Synonymous: 42 | Nonsynonymous: 74 | Stop Codon: 0

Protein Information

Protein Length: 682 | Molecular Weight (kDa): 81.095

UniProt ID(s): Q8II98

PDB ID(s): None

Isoelectric Point: 9.35

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006693	Partial AB-hydrolase lipase domain
InterPro	IPR029058	Alpha/Beta hydrolase fold
PFam	PF04083	Partial AB-hydrolase lipase domain
Superfamily	SSF53474	Alpha/Beta hydrolase fold