Genome location: Pf3D7_11_v3:1,059,925..1,061,367(-)
Genome classification: Core
Product Description: deoxyuridine 5'-triphosphate nucleotidohydrolase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.1.23 (dUTP diphosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0004170 | dUTP diphosphatase activity |
| Function | GO:0042802 | identical protein binding |
| Function | GO:0000287 | magnesium ion binding |
| Process | GO:0006260 | DNA replication |
| Process | GO:0006226 | dUMP biosynthetic process |
| Process | GO:0046081 | dUTP catabolic process |
| Process | GO:0006399 | tRNA metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.21 | 0.90 |
| Trophozoite | expressed | 1.41 | 0.39 |
| Schizont | expressed | 0.24 | 0.86 |
| Gametocyte | expressed | 0.50 | 0.72 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1127100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.847 | MFS: 0 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8II92
"Best" AlphaFill ligand hit: DUT (deoxyuridine-5'-triphosphate, Local RMSD=0.35) with 1SM8 (Global RMSD=11.42)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.1.23 | dUTP diphosphatase | UDPKClNEMNaFEDTAdTTPEGTAdCTPdUTPdUMPdTMPdUDP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8II92 | Deoxyuridine 5'-triphosphate nucleotidohydrolase | Plasmodium falciparum | HOGENOM, OMA, OrthoDB, OrthoMCL, OrthoMCL BLAST | CHEMBL369937CHEMBL371690CHEMBL195997CHEMBL197290... |
Ortholog Group (OrthoMCL): OG6_101166
Most Similar Human Ortholog: H0YMP1
TM-align score: 0.74 | RMSD: 1.82
Seq Identity: 0.24 | Length: 92 / 173
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000128951 | deoxyuridine triphosphatase |
Protein Length: 173 | Molecular Weight (kDa): 19.573
PDB ID(s): 1VYQ, 2Y8C, 3T60, 3T64, 3T6Y, 3T70
Isoelectric Point: 6.97
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR008181 | Deoxyuridine triphosphate nucleotidohydrolase |
| InterPro | IPR029054 | dUTPase-like |
| InterPro | IPR033704 | dUTPase, trimeric |
| InterPro | IPR036157 | dUTPase-like superfamily |
| PFam | PF00692 | dUTPase-like |
| Superfamily | SSF51283 | dUTPase-like superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 47
Non-coding: 40 | Synonymous: 6 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 17544982 | Plasmodium falciparum dUTPase: studies on protein stability and binding ofdeoxyuridine derivatives. | Quesada-Soriano I, Musso-Buendia JA, ..., Garcia-Fuentes L | 10.1016/j.bbapap.2007.04.013 |
| 18586121 | Kinetic and thermodynamic characterization of dUTP hydrolysis by Plasmodiumfalciparum dUTPase. | Quesada-Soriano I, Leal I, ..., Garcia-Fuentes L | 10.1016/j.bbapap.2008.05.014 |