PF3D7_1127300

Genome location: Pf3D7_11_v3:1,063,077..1,064,882(-)

Genome classification: Core

Function and Localization

Product Description: tRNA (guanine-N(7)-)-methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 2.1.1.33 (tRNA (guanine(46)-N(7))-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0043527	tRNA methyltransferase complex
Function	GO:0008168	methyltransferase activity
Function	GO:0008176	tRNA (guanine-N7-)-methyltransferase activity
Process	GO:0036265	RNA (guanine-N7)-methylation
Process	GO:0030488	tRNA methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.23	0.89
Trophozoite	expressed	0.38	0.78
Schizont	possibly expressed	0.05	0.97
Gametocyte	not expressed	0.16	0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1127300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.985 | MFS: -1.218 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0921100)

Relative Growth Rate: 0.79 ± 0.23
Confidence: 4.31

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0921100)

Modification: Disrupted | RMgm-2579

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8II90

"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.51) with 2VDV (Global RMSD=1.98)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.33	tRNA (guanine46-N7)-methyltransferase	MethylthioinosinemethylthioadenosineS-InosylhomocysteineS-adenosyl-L-cysteinep-hydroxymercuribenzoateSpleen phosphodiesteraseS-adenosyl-L-homocysteineS-Adenosyl-D-homocysteineS-adenosyl-DL-homocysteine

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100919

Most Similar Human Ortholog: Q9UBP6

TM-align score: 0.82 | RMSD: 1.96

Seq Identity: 0.40 | Length: 240 / 293

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000037897	methyltransferase like 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	14	7	27
disruptive	2	14	18	41
missense	2	14	17	35

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	26	13	22
disruptive	5	62	20	77
missense	2	50	17	59

PlasmoDB Total SNPs: 94

Non-coding: 83 | Synonymous: 9 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 293 | Molecular Weight (kDa): 35.557

UniProt ID(s): Q8II90

PDB ID(s): None

Isoelectric Point: 9.65

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type
InterPro	IPR025763	tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryote
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF02390	tRNA (guanine-N-7) methyltransferase, Trmb type
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase