PF3D7_1127500

Genome location: Pf3D7_11_v3:1,067,055..1,071,027(+)

Genome classification: Core

Function and Localization

Product Description: protein disulfide-isomerase, putative

SignalP Peptide: MIIKFLIFNLLLYLFRCNT

# Transmembrane Domains: 1

EC Numbers: 5.3.4.1 (Protein disulfide-isomerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005783	endoplasmic reticulum
Function	GO:0003756	protein disulfide isomerase activity
Process	GO:0006457	protein folding
Process	GO:0034976	response to endoplasmic reticulum stress

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	possibly expressed	0.21	0.87
Schizont	possibly expressed	0.22	0.85
Gametocyte	expressed	0.72	0.60

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1127500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.339 | MFS: -2.769 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0920900)

Relative Growth Rate: 0.23 ± 0.13
Confidence: 5.46

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0920900)

Modification: Disrupted | RMgm-2577

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8II88

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.3.4.1	protein disulfide-isomerase	NEM16F16LOC14AsO43-CCF642MA3 018MA3 019acroleinneomycinpeptidesgenisteinkanamycin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533311

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	16	11	31
disruptive	4	27	15	33
missense	4	27	15	33

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	42	18	28
disruptive	12	54	38	98
missense	12	48	34	76

PlasmoDB Total SNPs: 214

Non-coding: 186 | Synonymous: 17 | Nonsynonymous: 10 | Stop Codon: 1

Protein Information

Protein Length: 424 | Molecular Weight (kDa): 51.186

UniProt ID(s): Q8II88

PDB ID(s): None

Isoelectric Point: 6.88

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR013766	Thioredoxin domain
InterPro	IPR036249	Thioredoxin-like superfamily
PFam	PF00085	Thioredoxin domain
PFam	PF13848
Superfamily	SSF52833	Thioredoxin-like superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
12368868	Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14.	Gardner MJ, Shallom SJ, ..., Fraser CM	10.1038/nature01094