PF3D7_1128300 (PFK11)

Genome location: Pf3D7_11_v3:1,097,880..1,103,771(-)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent 6-phosphofructokinase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.1.11 (6-phosphofructokinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005945	6-phosphofructokinase complex
Component	GO:0005829	cytosol
Function	GO:0003872	6-phosphofructokinase activity
Function	GO:0008443	phosphofructokinase activity
Process	GO:0006096	glycolytic process
Process	GO:0009749	response to glucose

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.98
Trophozoite	possibly expressed	0.10	0.94
Schizont	not expressed	0.03	0.99
Gametocyte	expressed	0.80	0.65

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1128300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.821 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0919900)

Relative Growth Rate: 1.01 ± 0.12
Confidence: 5.65

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0919900)

Modification: Disrupted | RMgm-2574

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8II80

"Best" AlphaFill ligand hit: 1GP (sn-glycerol-1-phosphate, Local RMSD=0.65) with 4RGQ (Global RMSD=22.34)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.1.11	6-phosphofructokinase	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_156271

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	14	69	51	116
disruptive	20	95	53	149
missense	18	90	50	129

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	24	152	76	111
disruptive	44	227	136	281
missense	29	190	120	209

PlasmoDB Total SNPs: 225

Non-coding: 158 | Synonymous: 44 | Nonsynonymous: 23 | Stop Codon: 0

Protein Information

Protein Length: 1570 | Molecular Weight (kDa): 183.967

UniProt ID(s): Q8II80

PDB ID(s): None

Isoelectric Point: 8.45

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000023	Phosphofructokinase domain
InterPro	IPR035966	Phosphofructokinase superfamily
PFam	PF00365	Phosphofructokinase domain
Superfamily	SSF53784	Phosphofructokinase superfamily