Genome location: Pf3D7_11_v3:1,107,772..1,110,152(+)
Genome classification: Core
Product Description: Fe-S cluster assembly factor HCF101, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0051539 | 4 iron, 4 sulfur cluster binding |
| Process | GO:0016226 | iron-sulfur cluster assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.31 | 0.85 |
| Trophozoite | expressed | 0.33 | 0.80 |
| Schizont | not expressed | 0.06 | 0.96 |
| Gametocyte | possibly expressed | 0.29 | 0.83 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1128500
Resistome Missense Mutations: E305K
Resistome Compounds with Missense Mutations: AN13762
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.126 | MFS: -3.032 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0919700)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8II78
"Best" AlphaFill ligand hit: ACP (adenosine-5'-[beta, gamma-methylene]triphosphate, Local RMSD=0.20) with 5AUP (Global RMSD=3.48)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100169
Most Similar Human Ortholog: Q9Y5Y2
TM-align score: 0.85 | RMSD: 2.62
Seq Identity: 0.30 | Length: 257 / 718
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000095906 | nucleotide binding protein 2 |
| ENSG00000103274 | nucleotide binding protein 1 |
| ENSG00000151413 | nucleotide binding protein like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 36 | 19 | 37 |
| disruptive | 34 | 97 | 67 | 123 |
| missense | 18 | 73 | 56 | 92 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 62 | 30 | 44 |
| disruptive | 78 | 224 | 89 | 227 |
| missense | 38 | 168 | 70 | 142 |
PlasmoDB Total SNPs: 108
Non-coding: 27 | Synonymous: 46 | Nonsynonymous: 35 | Stop Codon: 0
Protein Length: 718 | Molecular Weight (kDa): 84.787
UniProt ID(s): Q8II78
PDB ID(s): None
Isoelectric Point: 8.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002744 | MIP18 family-like |
| InterPro | IPR010376 | Gamma-butyrobetaine hydroxylase-like, N-terminal |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR033756 | Flagellum site-determining protein YlxH/ Fe-S cluster assembling factor NBP35 |
| InterPro | IPR034904 | Fe-S cluster assembly domain superfamily |
| PFam | PF01883 | MIP18 family-like |
| PFam | PF06155 | Gamma-butyrobetaine hydroxylase-like, N-terminal |
| PFam | PF10609 | Flagellum site-determining protein YlxH/ Fe-S cluster assembling factor NBP35 |
| Superfamily | SSF117916 | Fe-S cluster assembly domain superfamily |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 33740894 | The iron-sulfur scaffold protein HCF101 unveils the complexity of organellarevolution in SAR, Haptista and Cryptista. | Pyrih J, Zarsky V, ..., Tachezy J | 10.1186/s12862-021-01777-x |