Genome location: Pf3D7_11_v3:1,112,700..1,115,761(+)
Genome classification: Core
Product Description: GPI-anchor transamidase, putative
SignalP Peptide: MGIKIIIYIFFLSWAKWVCG
# Transmembrane Domains: 2
EC Numbers: 3.4.13.19 (Membrane dipeptidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0042765 | GPI-anchor transamidase complex |
| Function | GO:0003923 | GPI-anchor transamidase activity |
| Function | GO:0004197 | cysteine-type endopeptidase activity |
| Process | GO:0016255 | attachment of GPI anchor to protein |
| Process | GO:0034394 | protein localization to cell surface |
| Process | GO:0006508 | proteolysis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.00 | 1.00 |
| Trophozoite | possibly expressed | 0.07 | 0.95 |
| Schizont | possibly expressed | 0.09 | 0.93 |
| Gametocyte | expressed | 0.12 | 0.92 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1128700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.214 | MFS: -2.706 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0919500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8II76
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.13.19 | membrane dipeptidase | DTTEDTANO3-SCN-HCO3-SO42-1G244acetatecitratePro-Alacysteinebestatin... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101767
Most Similar Human Ortholog: A6NEM5
TM-align score: 0.75 | RMSD: 2.86
Seq Identity: 0.30 | Length: 273 / 493
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000142892 | phosphatidylinositol glycan anchor biosynthesis class K |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 15 | 9 | 31 |
| disruptive | 21 | 56 | 27 | 68 |
| missense | 14 | 48 | 22 | 55 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 32 | 20 | 30 |
| disruptive | 36 | 131 | 57 | 105 |
| missense | 20 | 103 | 43 | 64 |
PlasmoDB Total SNPs: 185
Non-coding: 142 | Synonymous: 19 | Nonsynonymous: 24 | Stop Codon: 0
Protein Length: 493 | Molecular Weight (kDa): 59.689
PDB ID(s): None
Isoelectric Point: 9.14
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001096 | Peptidase C13, legumain |
| InterPro | IPR028361 | GPI-anchor transamidase |
| PFam | PF01650 | Peptidase C13, legumain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34954848 | Persistence of Trypanosoma brucei as early procyclic forms and social motilityare dependent on glycosylphosphatidylinositol transamidase | Knusel S, Jenni A, ..., Roditi I | 10.1111/mmi.14873 |
| 12802054 | Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase thatattaches GPI-anchors to proteins | Hong Y, Ohishi K, ..., Kinoshita T | 10.1091/mbc.e02-12-0794 |
| 17475206 | Truncation of the caspase-related subunit (Gpi8p) of Saccharomyces cerevisiae GPItransamidase: dimerization revealed | Meitzler JL, Gray JJ, Hendrickson TL | 10.1016/j.abb.2007.03.035 |
| 12958211 | GPI transamidase of Trypanosoma brucei has two previously uncharacterized(trypanosomatid transamidase 1 and 2) and three common subunits | Nagamune K, Ohishi K, ..., Kinoshita T | 10.1073/pnas.1833260100 |