Genome location: Pf3D7_11_v3:1,128,223..1,131,206(+)
Genome classification: Core
Product Description: 26S proteasome regulatory subunit RPN7, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0000502 | proteasome complex |
| Function | GO:0004175 | endopeptidase activity |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Process | GO:0006508 | proteolysis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.60 | 0.73 |
| Trophozoite | expressed | 1.63 | 0.28 |
| Schizont | expressed | 0.87 | 0.56 |
| Gametocyte | expressed | 0.80 | 0.58 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1129200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.157 | MFS: -2.738 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0919000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8II71
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102079
Most Similar Human Ortholog: Q15008
TM-align score: 0.92 | RMSD: 2.40
Seq Identity: 0.32 | Length: 380 / 393
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000163636 | proteasome 26S subunit, non-ATPase 6 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 22 | 14 | 27 |
| disruptive | 2 | 9 | 9 | 27 |
| missense | 2 | 9 | 9 | 25 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 45 | 17 | 34 |
| disruptive | 5 | 21 | 24 | 80 |
| missense | 5 | 18 | 18 | 55 |
PlasmoDB Total SNPs: 153
Non-coding: 142 | Synonymous: 7 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 393 | Molecular Weight (kDa): 46.624
UniProt ID(s): Q8II71
PDB ID(s): None
Isoelectric Point: 6.75
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000717 | Proteasome component (PCI) domain |
| InterPro | IPR019585 | 26S proteasome regulatory subunit Rpn7/COP9 signalosome complex subunit 1 |
| InterPro | IPR035268 | 26S Proteasome non-ATPase regulatory subunit 6 |
| InterPro | IPR036390 | Winged helix DNA-binding domain superfamily |
| PFam | PF01399 | Proteasome component (PCI) domain |
| PFam | PF10602 | 26S proteasome regulatory subunit Rpn7/COP9 signalosome complex subunit 1 |
| Superfamily | SSF46785 | Winged helix DNA-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 20471955 | Dissection of the assembly pathway of the proteasome lid in Saccharomycescerevisiae | Fukunaga K, Kudo T, ..., Saeki Y | 10.1016/j.bbrc.2010.05.061 |
| 15102831 | Rpn7 Is required for the structural integrity of the 26 S proteasome ofSaccharomyces cerevisiae | Isono E, Saeki Y, Yokosawa H, Toh-e A | 10.1074/jbc.M314231200 |
| 17761670 | Isolation of the Schizosaccharomyces pombe proteasome subunit Rpn7 and astructure-function study of the proteasome-COP9-initiation factor domain | Sha Z, Yen HC, ..., Chang EC | 10.1074/jbc.M706276200 |
| 17135287 | The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome | Isono E, Nishihara K, ..., Toh-e A | 10.1091/mbc.e06-07-0635 |