Genome location: Pf3D7_11_v3:1,137,082..1,140,631(-)
Genome classification: Core
Product Description: RNA cytosine C(5)-methyltransferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.- (Methyltransferases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005730 | nucleolus |
| Component | GO:0005634 | nucleus |
| Function | GO:0009383 | rRNA (cytosine-C5-)-methyltransferase activity |
| Process | GO:0001510 | RNA methylation |
| Process | GO:0000470 | maturation of LSU-rRNA |
| Process | GO:0070475 | rRNA base methylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.42 | 0.80 |
| Trophozoite | expressed | 0.54 | 0.70 |
| Schizont | expressed | 0.06 | 0.97 |
| Gametocyte | expressed | 0.32 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1129400
Resistome Missense Mutations: G284D
Resistome Compounds with Missense Mutations: Suloctidil
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.043 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1445300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8II69
"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.48) with 5ZVG (Global RMSD=3.38)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.- | Ex: homocysteine S-methyltransferase | EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100487
Most Similar Human Ortholog: F5GYR3
TM-align score: 0.60 | RMSD: 2.70
Seq Identity: 0.31 | Length: 185 / 634
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000111641 | NOP2 nucleolar protein |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 31 | 16 | 37 |
| disruptive | 17 | 74 | 49 | 87 |
| missense | 12 | 67 | 43 | 76 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 63 | 22 | 41 |
| disruptive | 32 | 177 | 70 | 182 |
| missense | 24 | 142 | 50 | 125 |
PlasmoDB Total SNPs: 161
Non-coding: 121 | Synonymous: 26 | Nonsynonymous: 14 | Stop Codon: 0
Protein Length: 634 | Molecular Weight (kDa): 74.369
UniProt ID(s): Q8II69
PDB ID(s): None
Isoelectric Point: 9.33
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001678 | SAM-dependent methyltransferase RsmB/NOP2-type |
| InterPro | IPR011023 | Nop2p |
| InterPro | IPR018314 | Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p, conserved site |
| InterPro | IPR023267 | RNA (C5-cytosine) methyltransferase |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| PFam | PF01189 | SAM-dependent methyltransferase RsmB/NOP2-type |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |