Product Description: A/G-specific adenine glycosylase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.2.2.20 (DNA-3-methyladenine glycosylase I)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0034039 | 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity |
| Function | GO:0035485 | adenine/guanine mispair binding |
| Function | GO:0032357 | oxidized purine DNA binding |
| Function | GO:0000701 | purine-specific mismatch base pair DNA N-glycosylase activity |
| Process | GO:0006281 | DNA repair |
| Process | GO:0006284 | base-excision repair |
| Process | GO:0006298 | mismatch repair |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.13 | 0.94 |
| Trophozoite | expressed | 0.80 | 0.57 |
| Schizont | expressed | 0.12 | 0.92 |
| Gametocyte | expressed | 0.53 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1129500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.991 | MFS: 0.125 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0918700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0918700)
Modification: Disrupted | RMgm-2566
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A143ZYC4
"Best" AlphaFill ligand hit: ADE (adenine, Local RMSD=0.94) with 1WEI (Global RMSD=1.75)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.2.2.20 | DNA-3-methyladenine glycosylase I | CaCl2spermidineapurinic-DNA3-butyladenine3-ethyladenine3-Methyladenine3-benzyladenine3-propyladenineN-ethylmaleimidep-mercuribenzoateDouble-stranded DNAp-hydroxymercuribenzoate... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102506
Most Similar Human Ortholog: Q5T418
TM-align score: 0.81 | RMSD: 2.62
Seq Identity: 0.26 | Length: 198 / 611
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000132781 | mutY DNA glycosylase |
| ENSG00000288208 | novel protein |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 35 | 18 | 42 |
| disruptive | 16 | 82 | 43 | 124 |
| missense | 11 | 72 | 39 | 110 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 72 | 24 | 41 |
| disruptive | 30 | 213 | 73 | 146 |
| missense | 14 | 183 | 65 | 119 |
PlasmoDB Total SNPs: 141
Non-coding: 117 | Synonymous: 11 | Nonsynonymous: 12 | Stop Codon: 1
Protein Length: 611 | Molecular Weight (kDa): 72.609
UniProt ID(s): A0A143ZYC4
PDB ID(s): None
Isoelectric Point: 9.29
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003265 | HhH-GPD domain |
| InterPro | IPR011257 | DNA glycosylase |
| PFam | PF00730 | HhH-GPD domain |
| Superfamily | SSF48150 | DNA glycosylase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |