PF3D7_1129600

Genome location: Pf3D7_11_v3:1,144,744..1,148,981(+)

Genome classification: Core

Function and Localization

Product Description: phosphatidylinositol-4-phosphate 5-kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.1.68 (1-phosphatidylinositol-4-phosphate 5-kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0016307	phosphatidylinositol phosphate kinase activity
Function	GO:0016773	phosphotransferase activity, alcohol group as acceptor
Process	GO:0046488	phosphatidylinositol metabolic process
Process	GO:0046854	phosphatidylinositol phosphate biosynthetic process
Process	GO:0048015	phosphatidylinositol-mediated signaling

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.04	0.98
Trophozoite	not expressed	0.09	0.95
Schizont	possibly expressed	0.21	0.87
Gametocyte	expressed	1.21	0.53

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1129600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.223 | #Insertions: 5

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0918600)

Relative Growth Rate: 1.03 ± 0.40
Confidence: 3.24

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0918600)

Modification: Disrupted | RMgm-2565

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8II67

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.1.68	1-phosphatidylinositol-4-phosphate 5-kinase	ATPADPNEMEDTAPCMBGSSGZnSO4Cutsumheparincysteinesperminequercetin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_109640

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	14	57	39	78
disruptive	47	155	100	227
missense	22	122	84	191

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	22	119	47	83
disruptive	99	342	154	339
missense	42	264	134	259

PlasmoDB Total SNPs: 210

Non-coding: 24 | Synonymous: 100 | Nonsynonymous: 86 | Stop Codon: 0

Protein Information

Protein Length: 1338 | Molecular Weight (kDa): 159.459

UniProt ID(s): Q8II67

PDB ID(s): None

Isoelectric Point: 9.77

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003409	MORN motif
PFam	PF02493	MORN motif
Superfamily	SSF82185	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104