Genome location: Pf3D7_11_v3:1,168,333..1,171,161(+)
Genome classification: Core
Product Description: 26S protease regulatory subunit 6A, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.8 (Transferred entry: 5.6.1.5)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0000502 | proteasome complex |
| Component | GO:0008540 | proteasome regulatory particle, base subcomplex |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0004175 | endopeptidase activity |
| Process | GO:0045899 | positive regulation of RNA polymerase II transcription preinitiation complex assembly |
| Process | GO:0070682 | proteasome regulatory particle assembly |
| Process | GO:0006508 | proteolysis |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.28 | 0.86 |
| Trophozoite | expressed | 0.86 | 0.54 |
| Schizont | expressed | 0.59 | 0.65 |
| Gametocyte | expressed | 0.61 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1130400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.125 | MFS: -3.028 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0917900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8II60
"Best" AlphaFill ligand hit: 08T ([[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium, Local RMSD=0.02) with 3U5Z (Global RMSD=9.90)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.8 | proteasome ATPase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101915
Most Similar Human Ortholog: R4GNH3
TM-align score: 0.92 | RMSD: 2.46
Seq Identity: 0.71 | Length: 417 / 439
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000165916 | proteasome 26S subunit, ATPase 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 24 | 8 | 31 |
| disruptive | 0 | 8 | 4 | 14 |
| missense | 0 | 8 | 4 | 11 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 66 | 15 | 30 |
| disruptive | 1 | 21 | 16 | 36 |
| missense | 1 | 17 | 14 | 25 |
PlasmoDB Total SNPs: 101
Non-coding: 94 | Synonymous: 6 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 439 | Molecular Weight (kDa): 49.541
UniProt ID(s): Q8II60
PDB ID(s): None
Isoelectric Point: 4.82
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR003960 | ATPase, AAA-type, conserved site |
| InterPro | IPR005937 | 26S proteasome regulatory subunit P45-like |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR032501 | Proteasomal ATPase OB C-terminal domain |
| InterPro | IPR041569 | AAA ATPase, AAA+ lid domain |
| PFam | PF00004 | ATPase, AAA-type, core |
| PFam | PF16450 | Proteasomal ATPase OB C-terminal domain |
| PFam | PF17862 | AAA ATPase, AAA+ lid domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 25177858 | Differential expression of 26S proteasome subunits and functional activity during neonatal development | Claud EC, McDonald JA, ..., Petrof EO | 10.3390/biom4030812 |
| 33862083 | Conserved proline residues in the coiled coil-OB domain linkers of Rpt proteins facilitate eukaryotic proteasome base assembly | Cheng CL, Wong MK, Li Y, Hochstrasser M | 10.1016/j.jbc.2021.100660 |
| 37214989 | Molecular mechanisms for activation of the 26S proteasome | Lee D, Zhu Y, ..., Lu Y | 10.1101/2023.05.09.540094 |
| 24743594 | Autoregulation of the 26S proteasome by in situ ubiquitination | Jacobson AD, MacFadden A, ..., Liu CW | 10.1091/mbc.E13-10-0585 |
| 27428775 | An atomic structure of the human 26S proteasome | Huang X, Luan B, Wu J, Shi Y | 10.1038/nsmb.3273 |
| 30629175 | Deregulation of the 19S proteasome complex increases yeast resistance to 4-NQOand oxidative stress via upregulation of Rpn4- and proteasome-dependent stressresponsive genes | Karpov DS, Spasskaya DS, ..., Karpov VL | 10.1093/femsyr/foz002 |
| 29636472 | Structural mechanism for nucleotide-driven remodeling of the AAA-ATPase unfoldase in the activated human 26S proteasome | Zhu Y, Wang WL, ..., Mao Y | 10.1038/s41467-018-03785-w |