PF3D7_1130600

Genome location: Pf3D7_11_v3:1,176,282..1,180,695(+)

Genome classification: Core

Function and Localization

Product Description: tRNA:m(4)X modification enzyme TRM13, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.225 (tRNA:m(4)X modification enzyme)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0008175	tRNA methyltransferase activity
Process	GO:0030488	tRNA methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.25	0.88
Trophozoite	expressed	0.37	0.79
Schizont	expressed	0.10	0.94
Gametocyte	expressed	0.36	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1130600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.3 | #Insertions: 9

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8II58

"Best" AlphaFill ligand hit: GTP (guanosine-5'-triphosphate, Local RMSD=1.29) with 4KB6 (Global RMSD=9.01)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.225	tRNA:m4X modification enzyme	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103381

Most Similar Human Ortholog: Q9NUP7

TM-align score: 0.53 | RMSD: 4.68

Seq Identity: 0.23 | Length: 312 / 888

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000122435	tRNA methyltransferase 13 homolog

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	57	21	58
disruptive	23	130	69	168
missense	19	118	64	149

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	95	41	63
disruptive	47	279	107	230
missense	29	241	88	166

PlasmoDB Total SNPs: 305

Non-coding: 213 | Synonymous: 43 | Nonsynonymous: 49 | Stop Codon: 0

Protein Information

Protein Length: 888 | Molecular Weight (kDa): 105.762

UniProt ID(s): Q8II58

PDB ID(s): None

Isoelectric Point: 8.95

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007871	Methyltransferase TRM13
InterPro	IPR039044	tRNA:m(4)X modification enzyme Trm13
PFam	PF05206	Methyltransferase TRM13
Superfamily	N/A	N/A