PF3D7_1132000

Genome location: Pf3D7_11_v3:1,239,829..1,242,921(+)

Genome classification: Core

Function and Localization

Product Description: ubiquitin-like protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Component	GO:0005634	nucleus
Function	GO:0031593	polyubiquitin modification-dependent protein binding
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.69	0.68
Trophozoite	expressed	0.76	0.58
Schizont	expressed	0.71	0.58
Gametocyte	expressed	0.93	0.50

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1132000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.585 | MFS: -3.037 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0916400)

Relative Growth Rate: 0.82 ± 0.10
Confidence: 5.92

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0916400)

Modification: Disrupted | RMgm-2556

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8II45

"Best" AlphaFill ligand hit: NAP (nadp nicotinamide-adenine-dinucleotide phosphate, Local RMSD=1.31) with 1PZ0 (Global RMSD=22.81)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100933

Most Similar Human Ortholog: H0YDS0

TM-align score: 0.32 | RMSD: 5.41

Seq Identity: 0.33 | Length: 91 / 882

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000135018	ubiquilin 1
ENSG00000160803	ubiquilin 4
ENSG00000188021	ubiquilin 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	15	46	33	51
disruptive	19	111	83	193
missense	15	92	72	156

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	26	102	43	60
disruptive	46	327	151	366
missense	27	267	122	191

PlasmoDB Total SNPs: 162

Non-coding: 69 | Synonymous: 31 | Nonsynonymous: 62 | Stop Codon: 0

Protein Information

Protein Length: 882 | Molecular Weight (kDa): 101.123

UniProt ID(s): Q8II45

PDB ID(s): None

Isoelectric Point: 6.47

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR009060	UBA-like superfamily
InterPro	IPR015496	Ubiquilin
InterPro	IPR015940	Ubiquitin-associated domain
InterPro	IPR029071	Ubiquitin-like domain superfamily
PFam	PF00627	Ubiquitin-associated domain
Superfamily	SSF46934	UBA-like superfamily
Superfamily	SSF54236	Ubiquitin-like domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108