Genome location: Pf3D7_11_v3:1,270,935..1,273,096(+)
Genome classification: Core
Product Description: aquaglyceroporin
SignalP Peptide: N/A
# Transmembrane Domains: 6
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0009986 | cell surface |
| Component | GO:0048471 | perinuclear region of cytoplasm |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0008519 | ammonium transmembrane transporter activity |
| Process | GO:0051475 | glucosylglycerol transport |
| Process | GO:0009247 | glycolipid biosynthetic process |
| Process | GO:0015791 | polyol transport |
| Process | GO:0006833 | water transport |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.03 | 0.99 |
| Trophozoite | expressed | 0.12 | 0.92 |
| Schizont | expressed | 0.51 | 0.70 |
| Gametocyte | expressed | 0.29 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1132800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.151 | MFS: -2.791 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0915600)
Modification: Tagged | RMgm-790
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8WPZ6
"Best" AlphaFill ligand hit: PEE (dope, Local RMSD=0.04) with 2ZZ9 (Global RMSD=2.33)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O43315 | Aquaporin-9 | OMA, OrthoDB | US10730865, Example 1US10730865, Example 2US10730865, Example 3US10730865, Example 4... |
Ortholog Group (OrthoMCL): OG6_100415
Most Similar Human Ortholog: Q96PS8
TM-align score: 0.91 | RMSD: 2.02
Seq Identity: 0.34 | Length: 252 / 258
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000103569 | aquaporin 9 |
| ENSG00000143595 | aquaporin 10 |
| ENSG00000165272 | aquaporin 3 (Gill blood group) |
Protein Length: 258 | Molecular Weight (kDa): 28.297
PDB ID(s): None
Isoelectric Point: 7.28
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000425 | Major intrinsic protein |
| InterPro | IPR023271 | Aquaporin-like |
| PFam | PF00230 | Major intrinsic protein |
| Superfamily | SSF81338 | Aquaporin-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 113
Non-coding: 108 | Synonymous: 4 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11729204 | A single, bi-functional aquaglyceroporin in blood-stage Plasmodium falciparummalaria parasites. | Hansen M, Kun JF, Schultz JE, Beitz E | 10.1074/jbc.M110683200 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16889642 | Ammonia permeability of the aquaglyceroporins from Plasmodium falciparum,Toxoplasma gondii and Trypansoma brucei. | Zeuthen T, Wu B, ..., Beitz E | 10.1111/j.1365-2958.2006.05325.x |
| 17284593 | Aquaglyceroporin PbAQP during intraerythrocytic development of the malariaparasite Plasmodium berghei. | Promeneur D, Liu Y, ..., Kumar N | 10.1073/pnas.0610843104 |
| 18500352 | Crystal structure of the aquaglyceroporin PfAQP from the malarial parasitePlasmodium falciparum. | Newby ZE, O'Connell J 3rd, ..., Stroud RM | 10.1038/nsmb.1431 |
| 22326891 | Molar concentrations of sorbitol and polyethylene glycol inhibit the Plasmodiumaquaglyceroporin but not that of E. coli: involvement of the channel vestibules. | Song J, Almasalmeh A, Krenc D, Beitz E | 10.1016/j.bbamem.2012.01.025 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 29330527 | Aquaglyceroporin PbAQP is required for efficient progression through the liverstage of Plasmodium infection. | Promeneur D, Mlambo G, Agre P, Coppens I | 10.1038/s41598-017-18987-3 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |