Genome location: Pf3D7_11_v3:1,273,208..1,275,484(+)
Genome classification: Core
Product Description: glycine cleavage system H protein
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.10 | 0.95 |
| Trophozoite | possibly expressed | 0.50 | 0.71 |
| Schizont | possibly expressed | 0.10 | 0.94 |
| Gametocyte | not expressed | 0.17 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1132900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.967 | MFS: -2.008 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0915500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8II35
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100619
Most Similar Human Ortholog: H3BUH9
TM-align score: 0.76 | RMSD: 2.65
Seq Identity: 0.24 | Length: 106 / 200
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000140905 | glycine cleavage system protein H |
| ENSG00000260643 | novel protein |
| ENSG00000284512 | novel protein |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 83
Non-coding: 63 | Synonymous: 16 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 200 | Molecular Weight (kDa): 23.187
UniProt ID(s): Q8II35
PDB ID(s): None
Isoelectric Point: 5.42
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002930 | Glycine cleavage system H-protein |
| InterPro | IPR003016 | 2-oxo acid dehydrogenase, lipoyl-binding site |
| InterPro | IPR011053 | Single hybrid motif |
| InterPro | IPR033753 | Glycine cleavage system H-protein/Simiate |
| PFam | PF01597 | Glycine cleavage system H-protein/Simiate |
| Superfamily | SSF51230 | Single hybrid motif |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 20403390 | Validation of a modified method for Bxb1 mycobacteriophage integrase-mediatedrecombination in Plasmodium falciparum by localization of the H-protein of theglycine cleavage complex to the mitochondrion. | Spalding MD, Allary M, Gallagher JR, Prigge ST | 10.1016/j.molbiopara.2010.04.005 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 30995911 | Hospital-derived antibody profiles of malaria patients in Southwest India | Venkatesh A, Jain A, ..., Rathod PK | 10.1186/s12936-019-2771-5 |