Genome location: Pf3D7_11_v3:1,308,312..1,312,020(+)
Genome classification: Core
Product Description: RNA (uracil-5-)methyltransferase
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.1.1.35 (tRNA (uracil(54)-C(5))-methyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.48 | 0.77 |
| Trophozoite | expressed | 1.08 | 0.45 |
| Schizont | expressed | 0.88 | 0.54 |
| Gametocyte | expressed | 0.86 | 0.56 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1133800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.123 | MFS: -3.149 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0914600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8II27
"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.67) with 2JJQ (Global RMSD=4.81)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.35 | tRNA (uracil54-C5)-methyltransferase | NH4+tRNAPhespermidineputrescinenorleucineL-ethioninemature tRNANO2Ura-tRNAL-methionineribonuclease5-fluorouracilethylthioadenosine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100839
Most Similar Human Ortholog: H7C100
TM-align score: 0.72 | RMSD: 1.52
Seq Identity: 0.22 | Length: 123 / 857
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000099899 | tRNA methyltransferase 2 homolog A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 32 | 19 | 52 |
| disruptive | 24 | 61 | 58 | 131 |
| missense | 18 | 55 | 55 | 106 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 64 | 28 | 46 |
| disruptive | 43 | 163 | 111 | 210 |
| missense | 29 | 130 | 88 | 134 |
PlasmoDB Total SNPs: 144
Non-coding: 101 | Synonymous: 22 | Nonsynonymous: 19 | Stop Codon: 2
Protein Length: 857 | Molecular Weight (kDa): 101.56
UniProt ID(s): Q8II27
PDB ID(s): None
Isoelectric Point: 9.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR010280 | (Uracil-5)-methyltransferase family |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| InterPro | IPR030390 | RNA methyltransferase TrmA, active site |
| InterPro | IPR030391 | RNA methyltransferase TrmA, conserved site |
| PFam | PF05958 | (Uracil-5)-methyltransferase family |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |