Genome location: Pf3D7_11_v3:1,318,721..1,322,792(+)
Genome classification: Core
Product Description: heat shock protein 70
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0005524 | ATP binding |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0031072 | heat shock protein binding |
| Function | GO:0051787 | misfolded protein binding |
| Function | GO:0044183 | protein folding chaperone |
| Function | GO:0051082 | unfolded protein binding |
| Process | GO:0034620 | cellular response to unfolded protein |
| Process | GO:0051085 | chaperone cofactor-dependent protein refolding |
| Process | GO:0042026 | protein refolding |
| Process | GO:0009408 | response to heat |
| Process | GO:0006986 | response to unfolded protein |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.55 | 0.75 |
| Trophozoite | expressed | 1.63 | 0.31 |
| Schizont | expressed | 0.16 | 0.92 |
| Gametocyte | expressed | 0.97 | 0.52 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1134000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.182 | MFS: -2.879 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8II24
"Best" AlphaFill ligand hit: DTP (, Local RMSD=0.15) with 5F0X (Global RMSD=3.49)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P11021 | Endoplasmic reticulum chaperone BiP | Homo sapiens | OrthoDB | CHEMBL3576921Hexach-loropheneHexach-loropheneadenosine-derived inhibitor, 12... |
| P0DMV8 | Heat shock 70 kDa protein 1A | Homo sapiens | OrthoDB | Brimonidine ATPAdenosine Diphosphate (ADP)adenosine-derived inhibitor, 4adenosine-derived inhibitor, 7... |
| P54652 | Heat shock-related 70 kDa protein 2 | OrthoDB | CHEMBL4473449CHEMBL4519613CHEMBL4545529CHEMBL4545529... | |
| P11142 | Heat shock cognate 71 kDa protein | Homo sapiens | OrthoDB | CHEMBL258670cid_274127cid_274125Aenosine 5'-Diphosphate |
Ortholog Group (OrthoMCL): OG6_100423
Most Similar Human Ortholog: A0A7I2V2G2
TM-align score: 0.95 | RMSD: 1.65
Seq Identity: 0.65 | Length: 597 / 663
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000113013 | heat shock protein family A (Hsp70) member 9 |
Protein Length: 663 | Molecular Weight (kDa): 73.297
PDB ID(s): None
Isoelectric Point: 6.74
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR012725 | Chaperone DnaK |
| InterPro | IPR013126 | Heat shock protein 70 family |
| InterPro | IPR018181 | Heat shock protein 70, conserved site |
| InterPro | IPR029047 | Heat shock protein 70kD, peptide-binding domain superfamily |
| InterPro | IPR029048 | Heat shock protein 70kD, C-terminal domain superfamily |
| InterPro | IPR043129 | ATPase, nucleotide binding domain |
| PFam | PF00012 | Heat shock protein 70 family |
| Superfamily | SSF100920 | Heat shock protein 70kD, peptide-binding domain superfamily |
| Superfamily | SSF100934 | Heat shock protein 70kD, C-terminal domain superfamily |
| Superfamily | SSF53067 | ATPase, nucleotide binding domain |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 26 | 9 | 35 |
| disruptive | 10 | 13 | 9 | 33 |
| missense | 10 | 13 | 9 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 61 | 31 | 31 |
| disruptive | 12 | 30 | 25 | 71 |
| missense | 12 | 25 | 18 | 45 |
PlasmoDB Total SNPs: 80
Non-coding: 58 | Synonymous: 12 | Nonsynonymous: 10 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 24045696 | Proteomic analysis of detergent-resistant membrane microdomains in trophozoiteblood stage of the human malaria parasite Plasmodium falciparum. | Yam XY, Birago C, ..., Ponzi M | 10.1074/mcp.M113.029272 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 32929216 | Heat stress induced arginylation of HuR promotes alternative polyadenylation of Hsp70.3 by regulating HuR stability and RNA binding | Deka K, Saha S | 10.1038/s41418-020-00619-5 |
| 34680697 | Heat Shock Protein 70 Family in Response to Multiple Abiotic Stresses in the Silkworm | Fang SM, Zhang Q, ..., Yu QY | 10.3390/insects12100928 |
| 36777720 | Genome-wide analysis of Hsp40 and Hsp70 gene family in four cotton species provides insights into their involvement in response to Verticillium dahliae andabiotic stress | Zhou X, Su L, ..., Li H | 10.3389/fgene.2023.1120861 |
| 31773487 | Effects of abiotic stress on the expression of Hsp70 genes in Sogatella furcifera (Horvath) | Zhou C, Yang XB, ..., Jin DC | 10.1007/s12192-019-01053-4 |
| 33793918 | HSP70-3 Interacts with Phospholipase Ddelta and Participates in Heat Stress Defense | Song P, Jia Q, ..., Zhang Q | 10.1093/plphys/kiaa083 |