Genome location: Pf3D7_11_v3:1,374,293..1,377,710(-)
Genome classification: Core
Product Description: protein phosphatase PPM8, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004724 | magnesium-dependent protein serine/threonine phosphatase activity |
| Function | GO:0004722 | protein serine/threonine phosphatase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.19 | 0.90 |
| Trophozoite | expressed | 0.10 | 0.94 |
| Schizont | expressed | 0.42 | 0.73 |
| Gametocyte | expressed | 0.18 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1135100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.018 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0913400)
Modification: Tagged | RMgm-1082
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8II13
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101170
Most Similar Human Ortholog: H3BRB7
TM-align score: 0.71 | RMSD: 1.36
Seq Identity: 0.00 | Length: 27 / 689
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000164951 | pyruvate dehyrogenase phosphatase catalytic subunit 1 |
| ENSG00000172840 | pyruvate dehyrogenase phosphatase catalytic subunit 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 22 | 10 | 21 |
| disruptive | 29 | 42 | 23 | 59 |
| missense | 27 | 35 | 20 | 45 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 34 | 18 | 33 |
| disruptive | 35 | 103 | 64 | 123 |
| missense | 30 | 78 | 50 | 75 |
PlasmoDB Total SNPs: 104
Non-coding: 65 | Synonymous: 16 | Nonsynonymous: 23 | Stop Codon: 0
Protein Length: 689 | Molecular Weight (kDa): 79.932
UniProt ID(s): Q8II13
PDB ID(s): None
Isoelectric Point: 7.1
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001932 | PPM-type phosphatase domain |
| InterPro | IPR015655 | Protein phosphatase 2C family |
| InterPro | IPR036457 | PPM-type phosphatase domain superfamily |
| PFam | PF00481 | PPM-type phosphatase domain |
| Superfamily | SSF81606 | PPM-type phosphatase domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |
| 35083211 | Functional Characterization of 17 Protein Serine/Threonine Phosphatases inToxoplasma gondii Using CRISPR-Cas9 System | Liang QL, Nie LB, ..., Wang JL | 10.3389/fcell.2021.738794 |