PF3D7_1135900

Genome location: Pf3D7_11_v3:1,398,462..1,401,319(-)

Genome classification: Core

Function and Localization

Product Description: 3-oxo-5-alpha-steroid 4-dehydrogenase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 5

EC Numbers: 1.3.99.5 (3-oxo-5-alpha-steroid 4-dehydrogenase (acceptor))

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0016491	oxidoreductase activity
Process	GO:0042761	very long-chain fatty acid biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.10	0.95
Trophozoite	expressed	0.40	0.75
Schizont	expressed	0.57	0.66
Gametocyte	expressed	0.17	0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1135900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.123 | MFS: -3.073 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0912700)

Relative Growth Rate: 0.08 ± 0.08
Confidence: 6.46

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8II05

"Best" AlphaFill ligand hit: NDP (nadph dihydro-nicotinamide-adenine-dinucleotide phosphate, Local RMSD=0.77) with 7C83 (Global RMSD=0.95)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.3.99.5	3-oxo-5alpha-steroid 4-dehydrogenase (acceptor)	KCNEDTAHgCl2CuSO4ZnSO4MnSO4MK386FK143MK906estronecatechinPNU157706...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P18405	3-oxo-5-alpha-steroid 4-dehydrogenase 1		OrthoMCL BLAST	17-Oxaandrost-5-ene-3β-yl-benzoate (1)17-Oxaandrost-5-ene-3β-yl-p-fluorobenzoate (2)17-Oxaandrost-5-ene-3β-yl-p-chlorobenzoate (3)17-Oxaandrost-5-ene-3β-yl-p-bromobenzoate (4)...
P31213	3-oxo-5-alpha-steroid 4-dehydrogenase 2	Homo sapiens	OrthoMCL BLAST	Finasteride, 3Steroid, 9aSteroid, 9bSteroid, 10a...
P31214	3-oxo-5-alpha-steroid 4-dehydrogenase 2	Rattus norvegicus	OrthoMCL BLAST	FINASTERIDECHEMBL1778071CHEMBL277224FINASTERIDE...
P24008	3-oxo-5-alpha-steroid 4-dehydrogenase 1	Rattus norvegicus	OrthoMCL BLAST	EpristerideCHEMBL79036CHEMBL3349141CHEMBL201762...
B7Z4L2	cDNA, FLJ78895, highly similar to 3-oxo-5-alpha-steroid 4-dehydrogenase 1		OrthoMCL BLAST	Finasteride (3)17α-Acetoxyprogesterone (6)3,20-Dioxopregna-4,6-dien-17α-yl acetate (7)3,20-Dioxo-6,7-epoxypregn-4-ene-7α-yl acetate (8)...

Orthology Information

Ortholog Group (OrthoMCL): OG6_102127

Most Similar Human Ortholog: Q9NZ01

TM-align score: 0.94 | RMSD: 1.51

Seq Identity: 0.37 | Length: 292 / 296

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000099797	trans-2,3-enoyl-CoA reductase

Protein Information

Protein Length: 296 | Molecular Weight (kDa): 35.468

UniProt ID(s): Q8II05

PDB ID(s): None

Isoelectric Point: 9.84

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal
InterPro	IPR029071	Ubiquitin-like domain superfamily
InterPro	IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase
PFam	PF02544	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal
Superfamily	SSF54236	Ubiquitin-like domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	16	11	16
disruptive	2	6	5	15
missense	2	5	5	13

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	36	13	21
disruptive	4	19	37	71
missense	2	11	32	51

PlasmoDB Total SNPs: 172

Non-coding: 156 | Synonymous: 13 | Nonsynonymous: 3 | Stop Codon: 0