Genome location: Pf3D7_11_v3:1,421,786..1,426,832(+)
Genome classification: Core
Product Description: tudor staphylococcal nuclease
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.31.1 (Micrococcal nuclease)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0005509 | calcium ion binding |
| Function | GO:0004518 | nuclease activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0006401 | RNA catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.66 | 0.71 |
| Trophozoite | expressed | 1.40 | 0.35 |
| Schizont | expressed | 0.78 | 0.58 |
| Gametocyte | expressed | 0.86 | 0.55 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1136300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.071 | #Insertions: 4
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0912300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0912300)
Modification: Disrupted | RMgm-2537
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8II01
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.31.1 | micrococcal nuclease | SDSEDTAcaspase-3Mononucleotidesoligonucleotidesmetacaspase mcII-Paadenosine 3',5'-diphosphatethymidine 3',5'-bisphosphate3',5'-deoxythymidine diphosphatedeoxythymidine 3',5'-diphosphatedeoxythymidine 3',5'-bisphosphate2'-deoxythymidine 3',5'-diphosphate |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102097
Most Similar Human Ortholog: Q7KZF4
TM-align score: 0.62 | RMSD: 5.38
Seq Identity: 0.23 | Length: 689 / 1098
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000197157 | staphylococcal nuclease and tudor domain containing 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 74 | 44 | 69 |
| disruptive | 19 | 110 | 76 | 179 |
| missense | 15 | 96 | 71 | 153 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 26 | 146 | 56 | 76 |
| disruptive | 48 | 271 | 172 | 337 |
| missense | 30 | 223 | 139 | 212 |
PlasmoDB Total SNPs: 301
Non-coding: 182 | Synonymous: 76 | Nonsynonymous: 43 | Stop Codon: 0
Protein Length: 1098 | Molecular Weight (kDa): 128.546
UniProt ID(s): Q8II01
PDB ID(s): None
Isoelectric Point: 8.9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002999 | Tudor domain |
| InterPro | IPR016071 | Staphylococcal nuclease (SNase-like), OB-fold |
| PFam | PF00565 | Staphylococcal nuclease (SNase-like), OB-fold |
| PFam | PF00567 | Tudor domain |
| Superfamily | SSF50199 | N/A |
| Superfamily | SSF63748 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 18035357 | Tudor domain proteins in protozoan parasites and characterization of Plasmodiumfalciparum tudor staphylococcal nuclease. | Hossain MJ, Korde R, ..., Malhotra P | 10.1016/j.ijpara.2007.09.018 |
| 20713134 | Plasmodium falciparum Tudor Staphylococcal Nuclease interacting proteins suggestits role in nuclear as well as splicing processes. | Hossain MJ, Korde R, ..., Malhotra P | 10.1016/j.gene.2010.08.004 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |