Genome location: Pf3D7_11_v3:1,431,881..1,435,170(-)
Genome classification: Core
Product Description: casein kinase 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0044538 | host cell periphery |
| Component | GO:0020009 | microneme |
| Component | GO:0005634 | nucleus |
| Function | GO:0005515 | protein binding |
| Function | GO:0004672 | protein kinase activity |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0006897 | endocytosis |
| Process | GO:0018105 | peptidyl-serine phosphorylation |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | nd | nd |
| Trophozoite | expressed | nd | nd |
| Schizont | expressed | nd | nd |
| Gametocyte | expressed | nd | nd |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1136500.2
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.225 | MFS: -2.966 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0912100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6S3F7
"Best" AlphaFill ligand hit: MIX (mitoxantrone, Local RMSD=0.24) with 2FUM (Global RMSD=3.16)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8N752 | Casein kinase I isoform alpha-like | Homo sapiens | HOGENOM, OrthoDB | Staurosporine, 8Staurosporine, 8 |
| P67827 | Casein kinase I isoform alpha | HOGENOM | CHEMBL3337846CHEMBL3337845 | |
| P48729 | Casein kinase I isoform alpha | Homo sapiens | HOGENOM, OMA, OrthoDB | BI-D1870Isoquinolinesulfonamide, CKI-7Epiblastin A6-(3-bromophenyl)pteridine-2,4,7-triamine (18)... |
| P78368 | Casein kinase I isoform gamma-2 | Homo sapiens | OrthoDB | US8759532, 1CHEMBL2071609CHEMBL2071610CHEMBL2071611... |
| Q06486 | Casein kinase I isoform delta | Rattus norvegicus | OrthoDB | 1-Acyl-1H-[1,2,4]triazole-3,5-diamine Analogue 3f1-Acyl-1H-[1,2,4]triazole-3,5-diamine Analogue 3ncid_1400cid_4878... |
| M3VH47 | Casein kinase 1, delta tv1 | OrthoDB | CHEMBL2295944CHEMBL2295959CHEMBL2295954CHEMBL2295956... | |
| Q9HCP0 | Casein kinase I isoform gamma-1 | Homo sapiens | OrthoDB | CHEMBL466496CHEMBL467079CHEMBL511410CHEMBL519113... |
| P97633 | Casein kinase I isoform alpha | Rattus norvegicus | OrthoDB | Meridianin BMeridianin E |
| P48730 | Casein kinase I isoform delta | OrthoDB | Epiblastin A6-(3-bromophenyl)pteridine-2,4,7-triamine (18)6-(1-benzothiophen-3-yl)pteridine-2,4,7-triamine (31)7-benzyl-6-(3-phenoxyphenyl)pteridine-2,4-diamine (36)... | |
| P49674 | Casein kinase I isoform epsilon | OrthoDB | Epiblastin A6-(2,4-dichlorophenyl)pteridine-2,4,7-triamine (25)6-(pyridin-2-yl)pteridine-2,4,7-triamine (30)6-(pyridin-2-yl)pteridine-2,4,7-triamine (30)... | |
| Q9Y6M4 | Casein kinase I isoform gamma-3 | Homo sapiens | OrthoDB | CHEMBL3337846CHEMBL3337845CHEMBL4101902Staurosporine, 8... |
Ortholog Group (OrthoMCL): OG6_100396
Most Similar Human Ortholog: B0QY36
TM-align score: 0.99 | RMSD: 0.69
Seq Identity: 0.72 | Length: 225 / 323
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000113712 | casein kinase 1 alpha 1 |
| ENSG00000141551 | casein kinase 1 delta |
| ENSG00000180138 | casein kinase 1 alpha 1 like |
| ENSG00000213923 | casein kinase 1 epsilon |
| ENSG00000283900 | TPTEP2-CSNK1E readthrough |
Protein Length: 323 | Molecular Weight (kDa): 37.631
UniProt ID(s): A0A144A308, Q8IHZ9
PDB ID(s): None
Isoelectric Point: 9.73
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR017441 | Protein kinase, ATP binding site |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 39 | 14 | 20 |
| disruptive | 0 | 2 | 2 | 35 |
| missense | 0 | 1 | 2 | 27 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 51 | 16 | 18 |
| disruptive | 2 | 23 | 21 | 66 |
| missense | 0 | 12 | 17 | 42 |
PlasmoDB Total SNPs: 65
Non-coding: 55 | Synonymous: 10 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 27923179 | Structural dynamics of Casein Kinase I (CKI) from malarial parasite Plasmodiumfalciparum (Isolate 3D7): Insights from theoretical modelling and molecularsimulations. | Dehury B, Behera SK, Mahapatra N | 10.1016/j.jmgm.2016.11.012 |
| 32356940 | Interaction of Plasmodium falciparum casein kinase 1 with components of host cellprotein trafficking machinery. | Batty MB, Schittenhelm RB, ..., Garcia-Bustos JF | 10.1002/iub.2294 |
| 38325718 | Casein kinase 1 inhibitor avoids TDP-43 pathology propagation in apatient-derived cellular model of amyotrophic lateral sclerosis | Cuevas EP, Martinez-Gonzalez L, ..., Martinez A | 10.1016/j.nbd.2024.106430 |
| 38023245 | Targeting casein kinase 1 for cancer therapy: current strategies and future perspectives | Long NH, Lee SJ | 10.3389/fonc.2023.1244775 |