Genome location: Pf3D7_11_v3:1,517,580..1,521,643(+)
Genome classification: Core
Product Description: protein phosphatase PPM2, protein phosphatase 2C
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004724 | magnesium-dependent protein serine/threonine phosphatase activity |
| Function | GO:0004722 | protein serine/threonine phosphatase activity |
| Process | GO:0006470 | protein dephosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.90 | 0.27 |
| Trophozoite | expressed | 1.44 | 0.33 |
| Schizont | expressed | 1.31 | 0.41 |
| Gametocyte | expressed | 1.20 | 0.41 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1138500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.19 | MFS: -2.797 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0910200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0910200)
Modification: Disrupted | RMgm-2524
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IHY0
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100270
Most Similar Human Ortholog: E9PKB5
TM-align score: 0.89 | RMSD: 2.32
Seq Identity: 0.33 | Length: 186 / 924
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000100614 | protein phosphatase, Mg2+/Mn2+ dependent 1A |
| ENSG00000115241 | protein phosphatase, Mg2+/Mn2+ dependent 1G |
| ENSG00000138032 | protein phosphatase, Mg2+/Mn2+ dependent 1B |
| ENSG00000285542 | novel protein, PPM1B-SLC3A1 readthrough |
Protein Length: 924 | Molecular Weight (kDa): 105.404
PDB ID(s): None
Isoelectric Point: 4.12
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000222 | PPM-type phosphatase, divalent cation binding |
| InterPro | IPR001932 | PPM-type phosphatase domain |
| InterPro | IPR015655 | Protein phosphatase 2C family |
| InterPro | IPR036457 | PPM-type phosphatase domain superfamily |
| PFam | PF00481 | PPM-type phosphatase domain |
| Superfamily | SSF81606 | PPM-type phosphatase domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 54 | 29 | 80 |
| disruptive | 32 | 96 | 76 | 162 |
| missense | 16 | 85 | 64 | 146 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 111 | 50 | 73 |
| disruptive | 49 | 263 | 134 | 307 |
| missense | 28 | 219 | 119 | 189 |
PlasmoDB Total SNPs: 173
Non-coding: 89 | Synonymous: 49 | Nonsynonymous: 35 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9556615 | Identification and characterization of an unusual double serine/threonine proteinphosphatase 2C in the malaria parasite Plasmodium falciparum. | Mamoun CB, Sullivan DJ Jr, Banerjee R, Goldberg DE | 10.1074/jbc.273.18.11241 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |