PF3D7_1139500

Genome location: Pf3D7_11_v3:1,576,132..1,578,978(-)

Genome classification: Core

Function and Localization

Product Description: AAA family ATPase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.21.92 (Endopeptidase Clp)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005694	chromosome
Component	GO:0005634	nucleus
Process	GO:0051598	meiotic recombination checkpoint signaling
Process	GO:0007131	reciprocal meiotic recombination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	nd	nd
Trophozoite	not expressed	nd	nd
Schizont	not expressed	nd	nd
Gametocyte	expressed	nd	nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1139500.2

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.669 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0909500)

Relative Growth Rate: 0.94 ± 0.09
Confidence: 6.31

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0909500)

Modification: Disrupted | RMgm-2519

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IHX1

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.12) with 6HD0 (Global RMSD=3.96)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.21.92	Endopeptidase Clp	NEMNeohydrincyclomarinrufomycin ICAANDENYALAAkappa-caseinphosphoarginineCAANDENYALAA-NH2Xaa-Tyr-Leu-Tyr-TrpHigh salt concentrationsdiisopropylfluorophosphatediisopropyl fluorophosphate...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103946

Most Similar Human Ortholog: Q15645

TM-align score: 0.88 | RMSD: 2.70

Seq Identity: 0.38 | Length: 414 / 645

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000071539	thyroid hormone receptor interactor 13

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	37	13	50
disruptive	8	52	30	70
missense	8	50	29	68

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	14	71	33	51
disruptive	17	95	57	143
missense	15	84	49	110

PlasmoDB Total SNPs: 176

Non-coding: 127 | Synonymous: 39 | Nonsynonymous: 10 | Stop Codon: 0

Protein Information

Protein Length: 645 | Molecular Weight (kDa): 75.148

UniProt ID(s): Q8IHX1

PDB ID(s): None

Isoelectric Point: 6.07

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001270	ClpA/B family
InterPro	IPR003593	AAA+ ATPase domain
InterPro	IPR003959	ATPase, AAA-type, core
InterPro	IPR003960	ATPase, AAA-type, conserved site
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00004	ATPase, AAA-type, core
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase