PF3D7_1139700

Genome location: Pf3D7_11_v3:1,582,539..1,585,322(-)

Genome classification: Core

Function and Localization

Product Description: adrenodoxin reductase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.18.1.2 (Ferredoxin--NADP(+) reductase);1.18.1.6 (Adrenodoxin-NADP(+) reductase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0015039	NADPH-adrenodoxin reductase activity
Function	GO:0016491	oxidoreductase activity
Process	GO:0055114	oxidation-reduction process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.33	0.86
Trophozoite	possibly expressed	0.26	0.83
Schizont	possibly expressed	0.14	0.90
Gametocyte	not expressed	0.07	0.96

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1139700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -3.079 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0909300)

Relative Growth Rate: 0.10 ± nan
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IHW9

"Best" AlphaFill ligand hit: NDP (nadph dihydro-nicotinamide-adenine-dinucleotide phosphate, Local RMSD=0.13) with 5JCA (Global RMSD=2.91)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.18.1.2	ferredoxin-NADP+ reductase	BRENDA SOAP query unsuccessful
1.18.1.6	adrenodoxin-NADP+ reductase	2',5'-ADPdiethyl dicarbonate5,5'-dithiobis(2-nitrobenzoate)

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101920

Most Similar Human Ortholog: J3QKZ8

TM-align score: 0.89 | RMSD: 1.81

Seq Identity: 0.33 | Length: 159 / 505

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000161513	ferredoxin reductase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	24	15	36
disruptive	2	27	27	47
missense	2	26	27	43

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	49	16	47
disruptive	8	64	46	101
missense	6	53	40	79

PlasmoDB Total SNPs: 110

Non-coding: 81 | Synonymous: 26 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 505 | Molecular Weight (kDa): 59.986

UniProt ID(s): Q8IHW9

PDB ID(s): None

Isoelectric Point: 9.81

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR021163	Ferredoxin-NADP+ reductase, adrenodoxin-type
PFam	N/A	N/A
Superfamily	SSF51971	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21