PF3D7_1140300

Genome location: Pf3D7_11_v3:1,604,250..1,607,491(+)

Genome classification: Core

Function and Localization

Product Description: P-loop containing nucleoside triphosphate hydrolase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.1.15 (Nucleoside-triphosphate phosphatase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	possibly expressed	0.08	0.94
Schizont	possibly expressed	0.06	0.96
Gametocyte	expressed	0.43	0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1140300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.613 | MFS: -3.206 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0908700)

Relative Growth Rate: 0.90 ± 0.06
Confidence: 6.95

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0908700)

Modification: Disrupted | RMgm-2514

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IHW2

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.1.15	nucleoside-triphosphate phosphatase	ATPADPNaFRNAEDTAdATPdTTPdCTPdGTPHOClrNTPMgCl2...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532862

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	18	12	22
disruptive	7	27	13	43
missense	6	27	13	38

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	36	18	28
disruptive	12	57	38	88
missense	9	49	31	56

PlasmoDB Total SNPs: 155

Non-coding: 140 | Synonymous: 2 | Nonsynonymous: 12 | Stop Codon: 1

Protein Information

Protein Length: 361 | Molecular Weight (kDa): 42.756

UniProt ID(s): Q8IHW2

PDB ID(s): None

Isoelectric Point: 8.56

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR030547	DNA repair protein XRCC2
PFam	N/A	N/A
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase