PF3D7_1141400 (PIGH)

Genome location: Pf3D7_11_v3:1,663,373..1,665,749(+)

Genome classification: Core

Function and Localization

Product Description: phosphatidylinositol N-acetylglucosaminyltransferase subunit H, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: 2.4.1.198 (Phosphatidylinositol N-acetylglucosaminyltransferase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.11	0.95
Trophozoite	expressed	0.96	0.51
Schizont	expressed	0.53	0.70
Gametocyte	expressed	0.53	0.71

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1141400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.226 | MFS: -2.53 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0907600)

Relative Growth Rate: 0.19 ± 0.11
Confidence: 5.75

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IHV1

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.4.1.198	phosphatidylinositol N-acetylglucosaminyltransferase	iodoacetic acidN-ethylmaleimideSH-alkylating reagentsp-chloromercuriphenylsulfonic acid

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105076

Most Similar Human Ortholog: Q14442

TM-align score: 0.64 | RMSD: 4.02

Seq Identity: 0.23 | Length: 163 / 353

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000100564	phosphatidylinositol glycan anchor biosynthesis class H

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	19	14	21
disruptive	12	49	24	64
missense	10	42	21	53

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	38	14	26
disruptive	24	116	41	94
missense	19	86	34	63

PlasmoDB Total SNPs: 113

Non-coding: 74 | Synonymous: 23 | Nonsynonymous: 15 | Stop Codon: 1

Protein Information

Protein Length: 353 | Molecular Weight (kDa): 42.183

UniProt ID(s): Q8IHV1

PDB ID(s): None

Isoelectric Point: 7.27

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR019328	GPI-GlcNAc transferase complex, PIG-H component, conserved domain
PFam	PF10181	GPI-GlcNAc transferase complex, PIG-H component, conserved domain
Superfamily	N/A	N/A