Genome location: Pf3D7_11_v3:1,668,302..1,669,090(-)
Genome classification: Core
Product Description: dolichol-phosphate mannosyltransferase
SignalP Peptide: MVIRFFLFVITLLGLCINMVCC
# Transmembrane Domains: 1
EC Numbers: 2.4.1.83 (Dolichyl-phosphate beta-D-mannosyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0005789 | endoplasmic reticulum membrane |
| Function | GO:0004582 | dolichyl-phosphate beta-D-mannosyltransferase activity |
| Process | GO:0006506 | GPI anchor biosynthetic process |
| Process | GO:0019348 | dolichol metabolic process |
| Process | GO:0035269 | protein O-linked mannosylation |
| Process | GO:0006486 | protein glycosylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.05 | 0.98 |
| Trophozoite | expressed | 0.41 | 0.75 |
| Schizont | expressed | 0.10 | 0.94 |
| Gametocyte | expressed | 0.10 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1141600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.128 | MFS: -2.885 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IHU9
"Best" AlphaFill ligand hit: UDP (uridine-5'-diphosphate, Local RMSD=1.12) with 5EKE (Global RMSD=3.81)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.4.1.83 | dolichyl-phosphate beta-D-mannosyltransferase | ATPADPUDPAMPGTPGDPUTPNEMUMPGMPHgCl2CuSO4... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100818
Most Similar Human Ortholog: A0A804HJ93
TM-align score: 0.91 | RMSD: 1.60
Seq Identity: 0.51 | Length: 132 / 259
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000000419 | dolichyl-phosphate mannosyltransferase subunit 1, catalytic |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 11 | 11 | 15 |
| disruptive | 1 | 17 | 15 | 37 |
| missense | 1 | 17 | 14 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 28 | 17 | 18 |
| disruptive | 4 | 54 | 34 | 79 |
| missense | 4 | 45 | 25 | 47 |
PlasmoDB Total SNPs: 13
Non-coding: 3 | Synonymous: 7 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 259 | Molecular Weight (kDa): 30.101
UniProt ID(s): Q8IHU9
PDB ID(s): None
Isoelectric Point: 8.68
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001173 | Glycosyltransferase 2-like |
| InterPro | IPR029044 | Nucleotide-diphospho-sugar transferases |
| InterPro | IPR039528 | DPM1-like |
| PFam | PF00535 | Glycosyltransferase 2-like |
| Superfamily | SSF53448 | Nucleotide-diphospho-sugar transferases |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12117963 | Genes for glycosylphosphatidylinositol toxin biosynthesis in Plasmodiumfalciparum. | Delorenzi M, Sexton A, ..., Schofield L | 10.1128/IAI.70.8.4510-4522.2002 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 35028934 | Aspirin damages the cell wall of Saccharomyces cerevisiae by inhibiting the expression and activity of dolichol-phosphate mannose synthase 1 | Li M, Zhu P, ..., Shi P | 10.1002/1873-3468.14283 |
| 33255655 | Yil102c-A is a Functional Homologue of the DPMII Subunit of Dolichyl Phosphate Mannose Synthase in Saccharomyces cerevisiae | Pilsyk S, Perlinska-Lenart U, ..., Kruszewska JS | 10.3390/ijms21238938 |