Genome location: Pf3D7_11_v3:1,701,397..1,703,791(-)
Genome classification: Core
Product Description: coproporphyrinogen-III oxidase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.3.3.3 (Coproporphyrinogen oxidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004109 | coproporphyrinogen oxidase activity |
| Process | GO:0006782 | protoporphyrinogen IX biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.51 | 0.76 |
| Trophozoite | expressed | 0.64 | 0.64 |
| Schizont | expressed | 0.28 | 0.81 |
| Gametocyte | not expressed | 0.30 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1142400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -3.078 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0906600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0906600)
Modification: Disrupted | RMgm-2505
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IHU1
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.3.3.3 | coproporphyrinogen oxidase | FADFMNKClSDSGSHEDTAEGTAGSSGriboflaviniodoacetateL-ethioninePhenylglyoxal... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101984
Most Similar Human Ortholog: P36551
TM-align score: 0.65 | RMSD: 1.85
Seq Identity: 0.39 | Length: 310 / 470
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000080819 | coproporphyrinogen oxidase |
| ENSG00000285635 | novel transcript |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 30 | 17 | 35 |
| disruptive | 5 | 12 | 10 | 38 |
| missense | 0 | 10 | 9 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 60 | 27 | 38 |
| disruptive | 14 | 39 | 45 | 120 |
| missense | 3 | 24 | 39 | 81 |
PlasmoDB Total SNPs: 112
Non-coding: 90 | Synonymous: 17 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 470 | Molecular Weight (kDa): 56.343
PDB ID(s): None
Isoelectric Point: 9.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001260 | Coproporphyrinogen III oxidase, aerobic |
| InterPro | IPR036406 | Oxygen-dependent coproporphyrinogen III oxidase superfamily |
| PFam | PF01218 | Coproporphyrinogen III oxidase, aerobic |
| Superfamily | SSF102886 | Oxygen-dependent coproporphyrinogen III oxidase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 20006984 | Characterization of coproporphyrinogen III oxidase in Plasmodium falciparumcytosol. | Nagaraj VA, Prasad D, ..., Padmanaban G | 10.1016/j.parint.2009.12.001 |
| 26173178 | Deconvoluting heme biosynthesis to target blood-stage malaria parasites | Sigala PA, Crowley JR, Henderson JP, Goldberg DE | 10.7554/eLife.09143 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |