PF3D7_1142700

Genome location: Pf3D7_11_v3:1,709,869..1,712,608(-)

Genome classification: Core

Function and Localization

Product Description: methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.43 (Transferred entry: 2.1.1.354)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.33	0.84
Trophozoite	expressed	0.35	0.80
Schizont	expressed	0.07	0.96
Gametocyte	not expressed	0.24	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1142700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.128 | MFS: -2.892 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0906300)

Relative Growth Rate: 0.11 ± 0.04
Confidence: 7.78

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IHT8

"Best" AlphaFill ligand hit: COA (coenzyme a, Local RMSD=0.03) with 3Q9U (Global RMSD=6.35)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.43	histone-lysine N-methyltransferase	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105985

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	48	18	37
disruptive	44	134	50	118
missense	25	112	43	88

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	19	68	36	44
disruptive	79	236	97	202
missense	51	184	75	117

PlasmoDB Total SNPs: 169

Non-coding: 97 | Synonymous: 32 | Nonsynonymous: 40 | Stop Codon: 0

Protein Information

Protein Length: 615 | Molecular Weight (kDa): 71.889

UniProt ID(s): Q8IHT8

PDB ID(s): None

Isoelectric Point: 4.54

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002110	Ankyrin repeat
InterPro	IPR019410	Lysine methyltransferase
InterPro	IPR020683	Ankyrin repeat-containing domain
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
InterPro	IPR036770	Ankyrin repeat-containing domain superfamily
PFam	PF10294	Lysine methyltransferase
PFam	PF12796	Ankyrin repeat-containing domain
Superfamily	SSF48403	Ankyrin repeat-containing domain superfamily
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104